Human Epidermal Growth Factor (hEGF)

• 产品编号：CST-8916SC      品牌：CST       原厂货号：8916SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > EGF 表皮生长因子
• 应用分类：

描述：

在Cell Signaling Technology公司的重组人源EGF (hEGF)天冬氨酸971-精氨酸1023（编号NM_0011963）经在大肠杆菌中表达生产。重组人源EGF在N-氨基末端有一个甲基化，分子量为6353. DTT还原和非还原蛋白游离基团为6kDa多肽。 预期在重组人源EGF的N-氨基末端MNSDS由氨基酸测序验证。6 μg还原和非还原的重组人源EGF 由SDS-PAGE测定大于98%。所有批次产品纯度均大于98%。重组人源EGF的生物活性由MCF10A细胞增殖实验测定。其每个批次的再ED50在 0.10-0.60 ng/ml之间。每1 μg hEGF中所含内毒素小于0.01 ng。有载体：每1 μg hEGF由含有20 μg BSA的pH 7.2的PBS 0.22微米过滤冻干。无载体：pH 7.2的PBS 0.22微米过滤冻干。EGF存在于上皮细胞，成纤维细胞和许多其他的细胞类型中（1,2）。低分子量可溶型EGF由一个分子量超过130,000的跨膜前体蛋白经蛋白水解生成（1,2）。可溶型和跨膜EGF都是有活性的（2）。EGF可以诱导多种细胞包括肿瘤来源的细胞增殖，分化和存活（1-3）。EGF家族和ErbB/HER EGF受体家族有许多成员。EGF结合ErbB1/HER1诱导同源二聚化或与其他ErbB/HER成员的异源二聚化（1）。EGF，EGF家族成员，EGF受体和它们的信号通路涉及到多种癌症，可以作为介入治疗的靶点（1,2）。

原厂资料：

Source / Purification

Recombinant human EGF (hEGF) Asn971-Arg1023 (Accession #NM_0011963) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hEGF has a Met on the amino terminus and has a calculated MW of 6353. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MNSDS of recombinant hEGF was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEGF. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hEGF was determined in a MCF10A cell proliferation assay. The ED50 of each lot is between 0.10-0.60 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hEGF.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hEGF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

EGF is produced by epithelial cells, fibroblasts, and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1-3). There are multiple members of the EGF family and multiple members of the ErbB/HER EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other ErbB/HER members (1).  Binding of EGF signals through the MAPK, PI3K/Akt, and Stat5 pathways (1). EGF, EGF family members, EGF receptors, and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.