Human Transforming Growth Factor β1 (hTGF-β1)

• 产品编号：CST-8915LC      品牌：CST       原厂货号：8915LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > TGF-beta 转化生长因子beta超家族
• 应用分类：

描述：

重组人源蛋白TGF-β1 (hTGF-β1)丙氨酸279-丝氨酸390（编号P01137）表达于Cell Signaling Technology公司的人293细胞中。人源重组TGF- β1不含标签蛋白这种非糖基化蛋白分子量为12794，DTT还原蛋白为13kDa多肽，未还原的由胱氨酸连接而成的二聚体分子量为25kDa。人源重组TGF- β1的预期N-氨基末端的ALDTN由氨基酸测序验证。6 μg还原和未还原的人源重组TGF-β1由SDS-PAGE测定纯度大于98%，在非还原条件下少于1%游离物hTGF-β1以单体形式存在。所有批次的产品纯度均大于98%。通过评估由抑制IL-4诱导的HT-2细胞的增殖来测定人源重组TGF- β1的生物活性。其每个批次的ED50在 0.04-0.20 ng/ml之间。每1 μg hTGF-β1溶于含有20 mM柠檬酸盐、100mM氯化钠、20 μgBSA、pH3.0的经0.22 μm滤器过滤的溶液后冻干带有载体。经0.22 μm滤器过滤的20 mM柠檬酸盐、100mM氯化钠、pH3.0的溶液的冻干物无载体。TGF-β1能激活包括增殖、血管生成和促进或抑制免疫在内的各种反应[1-3]。TGF-β1由各种细胞产生，包括调控性T淋巴细胞、成纤维细胞、上皮细胞和内皮细胞[3]。TGF-β1与TβRII二聚体结合形成复合体后又与TβRI二聚体结合[1,4]。此低聚物受体复合物磷酸化Smad蛋白小分子集团后能诱导或抑制一系列基因靶标[1,3,4]。TGF-β1的结合也可通过TAK1激活Erk2, p38和JNK 通路[4]。TGF-β1还被发现在促进某些癌症晚期转移和进展[1,2]。

原厂资料：

Source / Purification

Recombinant human TGF-β1 (hTGF-β1) Ala279-Ser390 (Accession #P01137) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hTGF-β1 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,794. DTT-reduced protein migrates as a 13 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 25 kDa protein. The expected amino-terminal ALDTN of recombinant hTGF-β1 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hTGF-β1. Less than 1% migrates as monomer hTGF-β1 under non-reduced (-) conditions. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hTGF-β1 was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. The ED50 of each lot is between 0.04-0.20 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hTGF-β1.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hTGF-β1. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.

Background

TGF-β1 activities include proliferation, angiogenesis and promotion or inhibition of many immune events (1-3). TGF-β1 is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells (3). TGF-β1 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,4). The oligomeric receptor complex phosphorylates subsets of the SMAD proteins that then act to induce and repress a number of target genes (1,3,4). TGF-β1 binding can also activate the Erk2, p38, and JNK pathways via TAK1 (4). TGF-β1 appears to promote late stage progression and metastasis in some cancers (1,2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.