Human Basic Fibroblast Growth Factor (hFGF basic/FGF2)

• 产品编号：CST-8910LF      品牌：Cell Signaling Technology       原厂货号：8910LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > FGF 成纤维细胞生长因子
• 应用分类：

描述：

Cell Signaling Technology公司生产的重组人源碱性成纤维细胞因子（hFGF basic）脯氨酸143-丝氨酸288（编号NP_001997）在大肠杆菌中表达。经过氨基酸测序得到，60-80%重组人源碱性FGF起始于N-氨基末端脯氨酸143（PALPE）分子量为16,539。其他剩余部分丢失脯氨酸143，起始于丙氨酸144（ALPED）。DTT还原和非还原的蛋白游离体位分子量17kDa多肽。6 μg还原和非还原的重组人源FGF 由SDS-PAGE测定大于98%。所有批次产品纯度均大于98%。重组人源碱性FGF的生物活性由NIH/3T3细胞增殖实验测定。其每个批次的ED50在 0.2-1.0 ng/ml之间。每1 μg人源碱性FGF所含内毒素少于0.01ng。

有载体的：每1 μg hFGF由含有20 μg BSA和10mM DTT的pH 7.2的PBS 0.22微米过滤冻干得到。胱氨酸对于其生物活性并非必须。无载体：含 10mM DTT 的pH 7.2的PBS 溶液经过0.22微米过滤冻干得到。胱氨酸对于其生物活性并非必须。

胚胎和成体细胞均能生成成纤维细胞因子(FGF basic，FGF2)，成纤维细胞因子与各种疾病的发生相关，包括癌症和动脉粥样硬化（1）。FGF2涉及发育过程并且参与调控分化，增殖和转移（1-6）。FGF2是在细胞培养中维持胚胎干细胞的生长而不分化的重要调控因子。FGF2与硫酸肝素有高亲和性（1,2），并且这一结合是FGF2激活FGFR赖氨酸激酶的其中一个步骤。有4个不同的FGF受体，每一个都有多个剪切异构体。FGF2与多个，但不是全部的FGFRs有高亲和性。FGF basic向FGFR的信号传递包括ras-raf-MAPK, PLCγ/PKC, 和PI3K/AKT信号通路（1）。

原厂资料：

Source / Purification

Recombinant human FGF basic (hFGF basic) Pro143-Ser288 (Accession #NP_001997) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Based on amino acid sequencing, 60-80% of recombinant hFGF basic starts at the amino-terminal Pro143 (PALPE) and has a calculated MW of 16,539. The remainder is missing Pro143 and starts at Ala144 (ALPED). DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hFGF basic. All lots are greater than 98% pure.

ioactivity

The bioactivity of recombinant hFGF basic was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.2 - 1.0 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hFGF basic.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 10mM DTT and 20 μg BSA per 1 μg hFGF basic. Cystines are not required for bioactivity. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 10mM DTT. Cystines are not required for bioactivity.

Background

FGF basic (FGF2) is producted in both embryonic and adult cell types, and contributes to the pathogenesis of various diseases, including cancer and atherosclerosis (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and binding is a step in the FGF basic activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.