Human Vascular Endothelial Growth Factor-121 (hVEGF₁₂₁ )

• 产品编号：CST-8908SC      品牌：CST       原厂货号：8908SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > VEGF血管内皮生长因子
• 应用分类：

描述：

CST公司在人293细胞中生产重组人蛋白VEGF121 (hVEGF121) Ala207-Arg327 (Accession #NP_001020541.2)。重组的hVEGF121蛋白没有标签，未糖基化蛋白的分子量据推算为14,057Da。DTT-还原蛋白迁移时是作为14-22 kDa 的多肽而转移。在SDS-PAGE中不同性质是由于糖基化造成。未还原的蛋白通过胱氨酸形成二聚体并作为30-36 kDa 多肽而迁移。重组hVEGF121蛋白的N-末端APMAE的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hVEGF121通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hVEGF121的生物活性是通过HUVEC细胞的细胞增殖实验确定的。每个批次的ED50在0.5-2 ng/ml之间。内毒素含量:<0.01 ng /1 μg hVEGF121。

有载体: 每微克hVEGF121蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hVEGF121蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

VEGF121是VEGF-A不同剪切中数量居第二的形式(1,2)。VEGF121是通过内皮细胞、巨噬细胞、T细胞和其它的一些细胞类型产生。VEGF121参与到了血管再生、血管内皮细胞生存、生长、迁移和血管渗透(1)。VEGF121的表达受到组织缺氧，炎症因子和肿瘤组织产生的致癌基因的诱导(1-3)。VEGF121结合到VEGFR1和VEGFR2 受体酪氨酸激酶(1)。VEGF121 结合到 VEGFR1和VEGFR2导致了信号通路的激活，如PI3K/AKT, P38 MAPK和 FAK (1)。在很多癌症中，VEGF在肿瘤血管再生中发挥了重要的作用(2)。

原厂资料：

Source / Purification

Recombinant human VEGF121 (hVEGF121) Ala207-Arg327 (Accession #NP_001020541.2) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hVEGF121 contains no "tags" and the nonglycosylated protein has a calculated MW of 14,057. DTT-reduced protein migrates as a 14-22 kDa polypeptide. Heterogeneity in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 30-36 kDa protein. The expected amino-terminal APMAE of recombinant hVEGF121 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hVEGF121. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hVEGF121 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 0.5-2 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg hVEGF121.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hVEGF121. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

VEGF121 is the second most abundant splice variant of VEGF-A (1,2). VEGF121 is produced by endothelial cells, macrophages, T-cells and other cell types. VEGF121 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1). VEGF121 expression is induced by hypoxia, inflammatory cytokines and through oncogene products in tumors (1-3). VEGF121 binds to VEGFR1 and VEGFR2 receptor tyrosine kinases (1). Binding of VEGF121 to VEGFR1 and VEGFR2 leads to activation of pathways involving PI3K/AKT, P38 MAPK, and FAK (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles