Human Interleukin-2 (hIL-2)

• 产品编号：CST-8907LC      品牌：CST       原厂货号：8907LC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-2 Family 白细胞介素-2超家族
• 应用分类：

描述：

CST公司在大肠杆菌中生产重组人蛋白IL-2 (hIL-2) Ala21-Thr153 (Accession #NM_000586)。重组的hIL-2蛋白在N-末端没有Met，其分子量据推算为15,418Da。DTT-还原和未还原的蛋白迁移时是作为一个14kDa 的多肽而转移，未还原的蛋白具有稍高的迁移率是由于内部二硫键的缘故。重组hIL-2蛋白的N-末端APTSS的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hIL-2通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hIL-2的生物活性是通过CTLL-2细胞增值实验确定的。每个批次的ED50在0.1 - 0.2 ng/ml之间。内毒素含量:<0.01 ng /1 μg hIL-2。

有载体: 每微克hIL-2蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-2蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-2是T细胞刺激细胞因子，因其对T细胞和NK细胞增殖的诱导和激活而知名(1,2)。IL-2也能促进外周Treg细胞的发育(3,4)。相反的，IL-2也参与到了激活对细胞死亡(AICD)的诱导中，此过程在后T细胞的扩充以增加 CD4+ T 细胞的Fas水平(5)。IL-2的效应是通过一个三聚体受体而介导，此三聚体包含IL-2Rα, IL-2Rβ和共有的γ链-γc (1,2)。 IL-2Rα特异性的以较低亲和度与 IL-2结合，而包括IL-2Rβ和γc亚单元的受体复合体结合亲和度明显提高。IL-15 也能结合到IL-2Rβ上 (1,2)。共有的γc链也被其它的细胞因子所利用，包括IL-4, IL-7, IL-9, IL-15和IL-21(1,2)。IL-2的结合起始了信号的级联反应，包括 Jak1, Jak3, Stat5和PI3K/Akt信号通路(1,2)。

原厂资料：

Source / Purification

Recombinant human IL-2 (hIL-2) Ala21-Thr153 (Accession #NM_000586) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-2 does not have a Met on the amino terminus and has a calculated MW of 15,418. DTT-reduced and non-reduced protein migrate as 14 kDa polypeptides with non-reduced protein having slightly greater mobility due to an intramolecular cystine. The expected amino-terminal APTSS of recombinant hIL-2 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-2. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-2 was determined in a CTLL-2 cell proliferation assay. The ED50 of each lot is between 0.1 - 0.2 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-2.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-2. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of Treg cells (4, 5). Conversely, IL-2 is involved in activation induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (3). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ and the common gamma chain, γc (1,2). IL-2 Rα binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-2Rβ is also used by IL-15 (1,2). The common γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5 and the PI3K/Akt pathways (1,2).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.