Human Interleukin-17F (hIL-17F)

• 产品编号：CST-8906SF      品牌：CST       原厂货号：8906SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-17 Family 白细胞介素-17超家族
• 应用分类：

描述：

CST公司生产的人重组蛋白 IL-17F (hIL-17F) Arg31-Gln163 (Accession #NP_443104)是从人的293细胞表达而来。重组的hIL-17F蛋白没有标签，未糖基化的蛋白分子量据推算为14,903Da。DTT-还原的蛋白分子作为22 kDa的蛋白而迁移。在SDS-PAGE中具稍低的迁移率是由于糖基化。未还原的蛋白由胱氨酸连接形成同源二聚体而作为38kDa 蛋白迁移。重组hIL-17F蛋白的N-末端RKIPK的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) 重组hIL-17F通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hIL-17F的生物活性是通过诱导3T3 MEFs WT产生IL-6能力的实验来确定的。每个批次的ED50在30-80 ng/ml之间。

有载体: 每微克hIL-17F蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-17F蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-17F是通过胱氨酸连接的同型二聚体，通过独特的CD4+ T细胞系TH17细胞产生的促炎细胞因子(1,2)。IL-17F诱导促炎因子，抗菌肽和中性粒细胞趋化剂如IL-6、β-defensin、IL-8、CXCL1 和 CXCL6 的产生(1,2)。这样就将天然免疫和获得性免疫连接起来。IL-17F高亲和性的结合到IL-17RC上，IL-17RC主要在非造血器官表达，而IL-17F与IL-17RA具有较低的亲和性，IL-17RA主要是在造血组织中表达(3)。IL-17F的结合会激活Erk1/2 MAP激酶和 NF-κB 信号通路(1,4)。IL-17F可能参与到了粘膜免疫细胞感染还很有可能与自体免疫条件有关(1,5,6)。

原厂资料：

Source / Purification

Recombinant human IL-17F (hIL-17F) Arg31-Gln163 (Accession #NP_443104) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-17F contains no "tags" and the nonglycosylated protein has a calculated MW of 14,903. DTT-reduced protein migrates as a 22 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 38 kDa protein. The expected amino-terminal RKIPK of recombinant hIL-17F was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17F. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-17F was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 30-80 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-17F.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-17F. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-17F is a cystine-linked homodimeric pro-inflammatory cytokine produced by TH17 cells, a distinct CD4+ T cell subset (1). IL-17F induces the production of pro-inflammatory cytokines, antimicrobial peptides, and neutrophil chemoattractants such as IL-6, β-defensin, IL-8, CXCL1, and CXCL6 (1,2). As a result, IL-17F may provide an important link between adaptive and the innate immunity. IL-17F binds with high affinity to IL-17RC, expressed primarily in non-hematopoietic tissues, and with lesser affinity to IL-17RA, which is expressed in hematopoietic tissues (3). IL-17F binding activates the ERK1/2 MAP kinase and NF-κB pathways (1,4). IL-17F appears to be involved in mucosal immunity against bacterial infections and possibly some autoimmune conditions (1,5,6).

注意事项：

Storage: Storage: Stable in lyophilized state at 4ºC for 1 year
after receipt. Sterile stock solutions reconstituted with carrier
protein are stable at 4ºC for 2 months and at -20ºC for 6 months.
Avoid repeated freeze-thaw cycles.