Human Interleukin-10 (hIL-10)

• 产品编号：CST-8903SC      品牌：CST       原厂货号：8903SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-10 Family 白细胞介素-10超家族
• 应用分类：

描述：

CST公司生产的人重组蛋白IL-10 (hIL-10) Ser19 - Asn178 (Accession # NM_000572) 是从大肠杆菌细胞表达而来。重组的hIL-10 蛋白在N-末端没有Met标签，蛋白分子量据推算为18,647Da。DTT-还原蛋白作为18kDa的多肽迁移，未还原蛋白由于有内部分子胱氨酸存在，作为16kDa的多肽迁移。重组hIL-10 蛋白的N-末端SPGQG的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) 重组hIL-10通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。hIL-10 的生物活性是通过MC/9细胞增殖实验来确定的。每个批次的ED50在 0.3-1.3 ng/ml之间。每微克hIL-10内毒素含量小于0.01 ng。

有载体：每微克mIFN-γ蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克mIFN-γ蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-10 是抗炎症的细胞因子，它由T细胞，NK细胞和巨噬细胞产生(1,2)。IL-10通过结合细胞表面受体复合体包括 IL-10 RI和 IL-10 RII而起始信号转导(1)。结合IL-10导致Jak1和Tyk2的激活, 继而磷酸化Stat-3 (1,3)。IL-10的抗炎症活性是由于它能通过其他的细胞因子受体而阻止细胞传递，特别是通过上调SOCS-1的表达而抑制IFNγ受体(1,3)。除此之外, IL-10通过抑制酪氨酸磷酸化CD28而促进T细胞耐受性(4,5)。IL-10是免疫响应的重要负调控因子,这能够维持怀孕状态(1)。相反,增加IL-10水平对利什曼虫的持续感染有很大的帮助(6)。

原厂资料：

Source / Purification

Recombinant human IL-10 (hIL-10) Ser19 - Asn178 (Accession # NM_000572) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-10 does not have a Met on the amino terminus and has a calculated MW of 18,647. DTT-reduced protein migrates as an 18 kDa polypeptide and non-reduced protein migrates as a 16 kDa polypeptide due to intramolecular cystines. The expected amino-terminal SPGQG of recombinant hIL-10 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-10. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-10 was determined in a MC/9 cell proliferation assay. The ED50 of each lot is between 0.3-1.3 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1μg hIL-10.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-10. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-10 is an anti-inflammatory cytokine that is produced by T cells, NK cells and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1). Binding of IL-10 leads to the activation of Jak1 and Tyk2, which phosphorylates Stat-3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFNγ receptor, by upregulating expression of SOCS-1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent Leishmania major infections (6).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.