Human Interleukin-1β (hIL-1β)

• 产品编号：CST-8900LF      品牌：CST       原厂货号：8900LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-1 Family 白细胞介素-1超家族
• 应用分类：

描述：

CST公司在大肠杆菌中生产重组人蛋白IL-1β (hIL-1β) Ala117-Ser269 (Accession #NM_000576)。重组的hIL-1β蛋白在N-末端没有Met，其分子量据推算为17,377Da。DTT-还原和未还原的蛋白迁移时是作为一个18kDa 的多肽而转移。重组hIL-1β蛋白的N-末端APVRS的序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) hIL-1β通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白IL-1β 的生物活性是通过诱导人的成纤维元代细胞产生IL-8的能力实验确定的。每个批次的ED50在5-20pg/ml之间。

有载体: 每微克hIL-1β蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-1β蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-1β是促炎症因子，主要的由激活的单核细胞和上皮细胞产生(1)。IL-1β的前体被caspase-1剪切，成熟的IL-1β紧接着被分泌(1-3)。目标细胞包括巨噬细胞和许多其它的细胞类型。IL-1β引起的信号通路包括IL-1β结合到IL-1的附属蛋白 (IL-1-AcP); 此复合体紧接着结合到IL-1RI (1,2)。信号通过激活MAP激酶和 NF-κB信号通路(1,2)。IL-1β也结合到IL-1RII,它缺乏细胞内的信号区域并因此作为高亲和性的诱捕受体。IL-1β结合到IL-1RI的过程能够被负调控因子IL-1R拮抗剂IL-1Ra所抑制。IL-1Ra结合到IL-1RI后无信号传导并且作为抑制剂阻止IL-1β信号通路。IL-1β在急性应答期和脓毒病中发挥了重要的作用(1-3)。

原厂资料：

Source / Purification

Recombinant human IL-1β (hIL-1β) Ala117-Ser269 (Accession #NM_000576) was produced in E. coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-1β does not have a Met on the amino terminus and has a calculated MW of 17,377. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal APVRS of recombinant hIL-1β was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-1β. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant IL-1β was determined by its ability to induce IL-8 production by primary human fibroblasts. The ED50 of each lot is between 5-20 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-1β.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-1β. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-1β is a pro-inflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.