Anti-rabbit IgG (H+L) F(ab)2 Fragment (Alexa Fluor? 594 Conjugate)

• 产品编号：CST-8889S      品牌：CST       原厂货号：8889S
• 产品分类：抗体 > 二抗 > 新型荧光染料标记
• 应用分类：

描述：

F(ab')2片段由山羊抗体制备，经混合的人和小鼠血清、浆细胞瘤/杂交瘤蛋白和纯化的人副蛋白类吸附。Anti-rabbit IgG (H+L), F(ab')2 Fragment在最适条件下与Alexa Fluor® 594荧光染料结合，产品规格为2 mg/ml。本F(ab')2 fragment通过Fc受体非特异性结合到细胞的情况较少。二抗的最佳稀释度根据实际应用来确定。最终稀释度为1：500时，对于大多数的免疫荧光分析都能得出可取的结果。荧光标记的抗物种IgG结合物是用于流式细胞术和免疫荧光的理想产品。CST公司严格的质量控制规程确保每个结合物都能提供最理想的特异性和荧光。

原厂资料：

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins.

Description

Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Applications

This product has been optimized for use as a secondary antibody in immunofluorescent applications.

Directions for Use

The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most immunofluorescent applications.

Background

Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

注意事项：

Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium
chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not
aliquot the antibody. Protect from light. Do not freeze.