Phospho-Survivin (Thr34) (D2E11) Rabbit mAb

• 产品编号：CST-8888S      品牌：CST       原厂货号：8888S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Survivin (Thr34) (D2E11) Rabbit mAb 兔单抗仅能够检测内源性水平的Thr34磷酸化的surviving蛋白。该单克隆抗体是采用合成的人源survivin蛋白Thr34周围残基相对应的肽段免疫动物而制备的。Survivin是一个在胎儿发育和肿瘤细胞恶变过程中高表达的抗凋亡蛋白，大小为16 kDa（1）。Survivin结合并抑制caspase-3，通过抑制细胞凋亡调控和促进细胞分裂，从而来控制细胞周期的G2/M-期临界点（2,3）。这个调控过程需要Survivin在P34 CDC2激酶的作用下，在Thr34位发生磷酸化（4）。通过基因打靶实验和Survivin反义RNA实验，应用Thr34磷酸化缺陷的survivin突变体，已经证明了survivin能够抑制肿瘤的生长（5,6）。

1. Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
2. Li, F. et al. (1998) Nature 396, 580-584.
3. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
5. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-Survivin (Thr34) (D2E11) Rabbit mAb recognizes endogenous levels of survivin protein only when phosphorylated at Thr34.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr34 of human survivin protein.

Background

Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6).

1. Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200.
2. Li, F. et al. (1998) Nature 396, 580-584.
3. Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466.
4. O'Connor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107.
5. Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809.
6. Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody