Human Interleukin-17A/F Heterodimer (hIL-17A/F)

• 产品编号：CST-8846SC      品牌：CST       原厂货号：8846SC
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-17 Family 白细胞介素-17超家族
• 应用分类：

描述：

重组的人蛋白IL-17A/F (hIL-17A/F)是通过GGGSGGGSGGGSGGGS连接的异源二聚体，此异源二聚体包含hIL-17A Ile20-Ala155 (Accession# NP_002181)和hIL-17F Arg31-Glu163 (Accession# NP_443104)。在CST公司hIL-17A/F在人的293细胞中表达。基于氨基酸序列, 多于80% 的重组hIL-17A/F 在Gly24 (GITIP)位点起始，其余的在Ile20 (IVKAG)位点起始。未糖基化的异源二聚体据推算分子量为31,396Da。DTT还原和未还原的蛋白作为38 kDa多肽而迁移。在SDS-PAGE中的低迁移率是由于糖基化。6 μg 还原 (+) 和 非还原的(-) hIL-17A/F通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白IL-17A/F的生物活性是通过对3T3 MEFs WT诱导产生IL-6实验确定的。每个批次的ED50在50-200 ng/ml之间。

有载体: 每微克hIL-17A/F蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-17A/F蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-17A/F是通过胱氨酸连接IL-17A和IL-17F的异型二聚体(1,2)。IL-17A/F是由Th17细胞和γδT细胞产生(1,2)。IL-17A/F 刺激促炎症因子和噬中性粒细胞趋化剂的产生, 因此作为先天免疫和获得性免疫的桥梁(1-3)。有一些研究表明IL-17A/F与粘膜免疫抵抗细菌侵染有密切的联系，同时也在自体免疫混乱过程中有潜在的功能(3,4)。IL-17A/F 的受体包含IL-17RA和IL-17RC异源二聚体，通过此受体的信号通路导致了Erk1/2 和 NF-κB信号通路的激活(1)。

原厂资料：

Source / Purification

Recombinant human IL-17A/F (hIL-17A/F) is a heterodimer that is composed of hIL-17A Ile20-Ala155 (Accession# NP_002181) linked to hIL-17F Arg31-Glu163 (Accession# NP_443104) via the linker GGGSGGGSGGGSGGGS. hIL-17A/F was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Based on amino acid sequencing, greater than 80% of recombinant hIL-17A/F starts at Gly24 (GITIP) and the remainder starts at Ile20 (IVKAG). The non-glycosylated heterodimer has a calculated MW of 31,396. The DTT-reduced and non-reduced protein migrate as 38 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation.

Purity>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17A/F. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-17 A/F was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 50-200 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-17A/F.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-17A/F. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.