Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb

• 产品编号：CST-8843S      品牌：CST       原厂货号：8843S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Horse

Specificity / Sensitivity

Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb recognizes endogenous levels of MOB1 protein only when phosphorylated at Thr12.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr12 of human MOB1 protein.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Horse

Specificity / Sensitivity

Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb recognizes endogenous levels of MOB1 protein only when phosphorylated at Thr12.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr12 of human MOB1 protein.

Background

MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy (1). Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and has been implicated in cancer development (2-5). There are two MOB1 proteins in humans, MOB1α and MOB1β, that are encoded by two different genes but have 96.3% identity (6). Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1 and 2 and NDR1 and 2, thereby stimulating kinase activity (7-9). This binding is promoted by the phosphorylation of MOB1 at several threonine residues by MST1 and/or MST2 (5,10).

Phosphorylation at Thr12 by MST1/2 stabilizes MOB1, enhancing its binding and regulation of LATS1 (5). The resultant increase in LATS1 kinase activity promotes inhibitory phosphorylation of the transcriptional co-activators YAP and TAZ (11,12), leading to changes in the expression of genes involved in cell cycle progression (13).

1. Luca, F.C. and Winey, M. (1998) Mol Biol Cell 9, 29-46.
2. Edgar, B.A. (2006) Cell 124, 267-73.
3. Saucedo, L.J. and Edgar, B.A. (2007) Nat Rev Mol Cell Biol 8, 613-21.
4. Harvey, K. and Tapon, N. (2007) Nat Rev Cancer 7, 182-91.
5. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
6. Praskova, M. et al. (2008) Curr Biol 18, 311-21.
7. Devroe, E. et al. (2004) J Biol Chem 279, 24444-51.
8. Hergovich, A. et al. (2005) Mol Cell Biol 25, 8259-72.
9. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
10. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.
11. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
12. Lei, Q.Y. et al. (2008) Mol Cell Biol 28, 2426-36.
13. Hao, Y. et al. (2008) J Biol Chem 283, 5496-509.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.