E-Cadherin (24E10) Rabbit mAb (Biotinylated)

• 产品编号：CST-8834S      品牌：CST       原厂货号：8834S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

E-Cadherin (24E10) Rabbit mAb (Biotinylated)用于检测内源性的E-钙粘素总蛋白。抗体不能与相关的家族成员例如N-钙粘素发生交叉反应。该单克隆抗体由人E-钙粘素780位脯氨酸附近的合成多肽免疫动物产生。这一CST抗体与生物素偶联。该生物素化的抗体可以用于人细胞的直接流式细胞检测。该抗体可以与未标记的E-Cadherin (24E10) Rabbit mAb #3195具有相同的种属交叉反应。钙粘素是胞外区重复大约100个残基的跨膜糖蛋白超家族。钙粘素介导钙相关的细胞-细胞粘附，并在正常组织发育中起重要作用(1)。经典的钙粘素超家族包括N-, P-, R-, B-, 和E-钙粘素，以及十多种在粘附连接处、极化的上皮细胞的顶点表面的细胞结构发现的其他成员。经典的钙粘素胞浆结构域与β-连环蛋白, γ-连环蛋白（也称为盘状球蛋白），以及p120连环蛋白相互作用。β-连环蛋白和γ-连环蛋白与α-连环蛋白相互作用，α-连环蛋白将钙粘素-连环蛋白复合物与肌动蛋白的细胞骨架相连(1,2)。β-连环蛋白和γ-连环蛋白在连接复合物中起结构性作用，p120则可以调节钙粘素的粘附活性和运输(1-4)。研究者认为E-钙粘素可以有效地抑制许多上皮细胞癌症的浸润和生长(1-3)。资金的研究表明癌细胞在E-钙粘素缺失的情况下可以上调N-钙粘素。这一钙粘素表达的改变被称为“钙粘素转变”。N-钙粘素与FGF受体相互作用，导致MMP-9的过度表达及细胞浸润(3)。研究表明，内皮细胞，VE-钙粘素信号传导、表达以及定位与血管渗透性和肿瘤血管发生有关(5,6)。研究者也提出，P-钙粘素通常表达于上皮细胞，并在卵巢或其他人类癌症中改变(7,8)。

1. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
2. Christofori, G. (2003) EMBO J 22, 2318-23.
3. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34.
5. Rabascio, C. et al. (2004) Cancer Res 64, 4373-7.
6. Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7.
7. Patel, I.S. et al. (2003) Int J Cancer 106, 172-7.
8. Sanders, D.S. et al. (2000) J Pathol 190, 526-30.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig

Specificity / Sensitivity

E-Cadherin (24E10) Rabbit mAb (Biotinylated) detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Pro780 of human E-cadherin protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated E-Cadherin (24E10) Rabbit mAb #3195.

Background

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

1. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
2. Christofori, G. (2003) EMBO J 22, 2318-23.
3. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34.
5. Rabascio, C. et al. (2004) Cancer Res 64, 4373-7.
6. Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7.
7. Patel, I.S. et al. (2003) Int J Cancer 106, 172-7.
8. Sanders, D.S. et al. (2000) J Pathol 190, 526-30.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA and 50%
glycerol. Store at -20ºC. Do not aliquot the antibody