GRAF1 Antibody

• 产品编号：CST-8802S      品牌：CST       原厂货号：8802S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GRAF1 Antibody兔多抗能够检测内源性的CPT1A总蛋白。该多克隆抗体是由合成的人源GRAF1蛋白赖氨酸(261位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。GTP酶调节相关的黏着斑激酶-1（GRAF1）是一种小G蛋白RhoA和CDC42 GTP酶激活蛋白（1）。它由N-末端BAR结构域，PH结构域，RhoGAP域，富含脯氨酸域和C-末端SH3结构域构成。 GRAF1作用于clathrin非依赖载体/ GPI富集的早期内涵体（CLIC/ GEEC）途径，是这种内吞途径中被发现的第一个特定蛋白质成分（2）。 GRAF1是腺相关病毒2型感染所必需的一种重要的蛋白质（3）。此外，研究已发现GRAF1与精神发育迟滞（4），骨骼肌分化（5）和髓细胞性白血病（6,7）相关。

1. Hildebrand, J.D. et al. (1996) Mol Cell Biol 16, 3169-78.
2. Lundmark, R. et al. (2008) Curr Biol 18, 1802-8.
3. Nonnenmacher, M. and Weber, T. (2011) Cell Host Microbe 10, 563-76.
4. Barresi, V. et al. (2010) BMC Med Genomics 3, 28.
5. Doherty, J.T. et al. (2011) J Biol Chem 286, 25903-21.
6. Qian, J. et al. (2011) Leuk Res 35, 783-6.
7. Qian, Z. et al. (2010) J Exp Clin Cancer Res 29, 111.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine, Horse

Specificity / Sensitivity

GRAF1 Antibody recognizes endogenous levels of total GRAF1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys261 of human GRAF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

GTPase Regulator Associated with Focal Adhesion Kinase-1 (GRAF1), is a GTPase-activating protein for the small G proteins RhoA and Cdc42 (1). It is composed of an N-terminal BAR domain, a PH domain, a RhoGAP domain, a proline-rich domain, and a C-terminal SH3 domain. GRAF1 contributes to the clathrin-independent carriers/GPI-enriched early endosomal compartments (CLIC/GEEC) pathway, and was the first specific protein component of this endocytic pathway to be discovered (2). GRAF1 was identified as an important protein necessary for adeno-associated virus 2 infection (3). In addition, research studies have linked GRAF1 to mental retardation (4), skeletal muscle differentiation (5), and myeloid leukemia (6,7).

1. Hildebrand, J.D. et al. (1996) Mol Cell Biol 16, 3169-78.
2. Lundmark, R. et al. (2008) Curr Biol 18, 1802-8.
3. Nonnenmacher, M. and Weber, T. (2011) Cell Host Microbe 10, 563-76.
4. Barresi, V. et al. (2010) BMC Med Genomics 3, 28.
5. Doherty, J.T. et al. (2011) J Biol Chem 286, 25903-21.
6. Qian, J. et al. (2011) Leuk Res 35, 783-6.
7. Qian, Z. et al. (2010) J Exp Clin Cancer Res 29, 111.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody