Human Interleukin-28B (hIL-28B/IFN-λ3)

• 产品编号：CST-8796LF      品牌：CST       原厂货号：8796LF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它白细胞介素
• 应用分类：

描述：

CST公司利用重组的人IL-28B (hIL-28B) Arg26-Val196 (Accession #NP_742151)在人的293细胞中表达。重组的hIL-28B蛋白没有标签，其未糖基化分子量据推算为19,264Da。DTT-还原和未还原的蛋白迁移时是作为一个21kDa 的多肽而转移，没有还原的蛋白由于内部胱氨酸分子内作用而具有稍高的迁移率。重组hIL-28B蛋白的N-末端序列RLRGA通过测序得到。6 μg 还原 (+) 和 非还原的(-) hIL-28B通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。重组蛋白hIL-28B的生物活性是通过病毒保护实验确定的。每个批次的ED50在0.4-9 ng/ml之间。内毒素含量:<0.01 ng/1 μg hIL-28B。

有载体: 每微克hIL-28B蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克hIL-28B蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

IL-28B是干扰素λ细胞因子家族成员之一,包括IL-29和IL-28A(1)。IL-28B在很多类型的细胞中产生并且与参与到了I型干扰素的很多功能中来(1-3)。IL-28B在体内和体外都存在抗病毒的活性(1,2)。很多的研究表明IL-28B在体内直接抑制肿瘤细胞的增殖并促进抗肿瘤免疫应答(1,3)。IL-28的受体是包含IL-28Rα和 IL-10RI的异源二聚体(1)。IL-28A激活Stat1, Stat3 和Stat5 (1)。IL-28Rα的表达只是局限在一小部分的细胞类型中,包括类浆细胞DC 和上皮细胞(1)。

原厂资料：

Source / Purification

Recombinant human IL-28B (hIL-28B) Arg26-Val196 (Accession #NP_742151) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-28B contains no "tags" and the nonglycosylated protein has a calculated MW of 19,264. DTT-reduced and non-reduced protein migrate as 21 kDa polypeptides with non-reduced protein having slightly greater mobility due to intramolecular cystines. The expected amino-terminal RLRGA of recombinant hIL-28B was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-28B. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-28B was determined in a virus protection assay. The ED50 of each lot is between 0.4-9 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-28B.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-28B. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-28B is a member of the Interferon λ family of cytokines, which includes IL-29 and IL-28A (1). IL-28B is produced by a number of cell types and shares many functions with Type I Interferons (1-3). IL-28B exhibits anti-viral activities in vitro and in vivo (1,2). Investigators have found that IL-28B directly inhibits tumor cell proliferation and promotes anti-tumor immune responses in vivo (1,3). The IL-28 receptor is a heterodimer of the IL-28Rα and IL-10RI (1). IL-28A activates Stat1, Stat3, and Stat5 (1). IL-28Rα expression is limited to a few cell types, including plasmacytoid DC and epithelial cells (1).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles.