NKX2.5 (E1Y8H) Rabbit mAb

• 产品编号：CST-8792S      品牌：CST       原厂货号：8792S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NKX2.5 (E1Y8H) Rabbit mAb兔单抗能识别内源性的NKX2.5总蛋白。此单克隆抗体通过合成肽免疫动物制备，这种合成肽与人源NKX2.5蛋白的Pro67邻近氨基酸序列相一致。NKX2.5是NKX同源盒转录因子家族的一员。NKX2.5在心脏发育过程中发挥了关键作用，也是胚胎发育时心脏中表达的早期因子之一。靶向缺失小鼠Nkx2.5基因会导致心脏形态异常，严重的发育迟缓以及E9.5胚胎致死(1,2)。NKX2.5突变也同样和几种先天性心脏病相关，例如房室传导缺损（ASD-AVCD）和法洛四联症（TOF）(3,4)。NKX2.5的转录激活也与染色体易位导致的某些B和T细胞白血病有关(5-8)。

1. Lyons, I. et al. (1995) Genes Dev 9, 1654-66.
2. Tanaka, M. et al. (1999) Development 126, 1269-80.
3. Benson, D.W. et al. (1999) J Clin Invest 104, 1567-73.
4. Reamon-Buettner, S.M. and Borlak, J. (2010) Hum Mutat 31, 1185-94.
5. Nagel, S. et al. (2003) Cancer Res 63, 5329-34.
6. Przybylski, G.K. et al. (2006) Haematologica 91, 317-21.
7. Nagel, S. et al. (2007) Cancer Res 67, 1461-71.
8. Su, X. et al. (2008) Haematologica 93, 1081-5.

原厂资料：

Specificity / Sensitivity

NKX2.5 (E1Y8H) Rabbit mAb recognizes endogenous levels of total NKX2.5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human NKX2.5 protein.

Background

NKX2.5 is a member of the NKX homeobox transcription factor family. NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine Nkx2.5 gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2). Mutations in NKX2.5 are likewise associated with several congenital heart conditions, such as atrial defect with atrioventricular conduction defects (ASD-AVCD) and Tetralogy of Fallot (TOF) (3,4). Transcriptional activation of NKX2.5 is also associated with some B and T cell leukemias that result from chromosomal translocation (5-8).

1. Lyons, I. et al. (1995) Genes Dev 9, 1654-66.
2. Tanaka, M. et al. (1999) Development 126, 1269-80.
3. Benson, D.W. et al. (1999) J Clin Invest 104, 1567-73.
4. Reamon-Buettner, S.M. and Borlak, J. (2010) Hum Mutat 31, 1185-94.
5. Nagel, S. et al. (2003) Cancer Res 63, 5329-34.
6. Przybylski, G.K. et al. (2006) Haematologica 91, 317-21.
7. Nagel, S. et al. (2007) Cancer Res 67, 1461-71.
8. Su, X. et al. (2008) Haematologica 93, 1081-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody