Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific Blue? Conjugate)

• 产品编号：CST-8788S      品牌：CST       原厂货号：8788S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific Blue™ Conjugate) 兔单抗仅能够检测内源性水平的Asp175磷酸化的Caspase-3蛋白。该单克隆抗体是采用合成的人源caspase-3蛋白Asp175周围残基相对应的肽段免疫动物而制备的。该CST抗体标记了Pacific Blue^TM 荧光染料，内部测试证明可以直接用于人细胞的免疫荧光试验。该抗体与不带标记的Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb兔单抗 #9579有着相同的种属交叉反应。Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者，因为它对许多关键的蛋白起着部分或全部的裂解作用，比如核酶多聚(ADP-ribose)聚合酶(PARP)（1）。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点（2）。

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Bovine, Pig

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue^TM fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze