ER-Tracker? Green (BODIPY? FL Glibenclamide)

• 产品编号：CST-8787S      品牌：CST       原厂货号：8787S
• 产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 细胞器染料
• 应用分类：

描述：

提供的ER-Tracker™ Green (BODIPY® FL Glibenclamide)是一个含有100μg冻干粉的小瓶。对于1mM贮存液，将冻干粉溶于128 μl高品质的DMSO。直接在普通培养基中以1:500稀释，工作浓度为2 μM，然后立即进行分析。细胞必须实时成像，不要固定！为了减少培养基的背景荧光，在成像前用没有酚红的培养基代替普通培养基。绿色背景抑制剂，如BackDrop™ Green Background Suppressor (Life Technologies, Carlsbad, CA)，也可以用来减少背景荧光。ER-Tracker™ Green (BODIPY® FL Glibenclamide)是一种细胞渗透剂、活细胞染色剂，对内质网（ER）有高度选择性。染色包括绿色荧光BODIPY® FL和格列本脲。格列本脲能结合磺脲受体，该受体是主要在ER中表达的ATP敏感的钾通道的调节亚基。格列本脲的药理学活性可能潜在影响ER的功能。磺脲受体在一些专门的细胞中的不同程度的表达可能导致非内质网标记。

原厂资料：

Directions for Use

ER-Tracker™ Green (BODIPY® FL Glibenclamide) is supplied as a single 100 μg lyophilized vial. To make a 1 mM stock solution, reconstitute the solid in 128 μl of high quality DMSO. Dilute 1:500 directly into normal growth media for a working concentration of 2 μM and analyze immediately. Cells must be imaged live; DO NOT FIX! 
 To reduce background fluorescence of the media, phenol-red free media may be substituted for normal media prior to imaging. A green background suppressor, such as BackDrop™ Green Background Suppressor (Life Technologies, Carlsbad, CA) can also be added to reduce background fluorescence.

Background

ER-Tracker™ Green (BODIPY® FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). This stain consists of the green-fluorescent BODIPY® FL dye and glibenclamide. Glibenclamide (glyburide) binds to sulphonylurea receptors, the regulatory subunit of ATP-sensitive K+ channels prominently expressed in the ER. The pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

注意事项：

Storage: Store lyophilized solid or solution at –20°C dessicated
and protected from light. Once reconstituted in DMSO, the
solution should be aliquoted to avoid freeze-thaw cycles. If stored
properly, this reagent is stable for 6 months.