Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated)

• 产品编号：CST-8785S      品牌：CST       原厂货号：8785S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nanog (D1G10) Rabbit mAb兔单抗 (小鼠特异; ChIP 级别)可免疫沉淀小鼠内源的总nanog蛋白，而与人源nanog蛋白没有交叉反应。此单克隆抗体由合成肽段免疫动物产生，该肽段与小鼠nanog蛋白邻近氨基末端的氨基酸序列一致。Nanog是一个含有同源结构域的转录因子，对于维持胚胎干细胞的多潜能性和自我更新非常重要（1）。Nanog的表达由包括Sox2和关键多潜能性调控因子Oct-4在内的调控网络调控（1）。近来对于体细胞重编程的研究进展主要通过整合包含nanog, Oct-4, Sox2, KLF4, c-Myc和 LIN28等转录因子病毒表达来实现 (2,3).

1. Kim, J. et al. (2008) Cell 132, 1049-61.
2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
3. Yu, J. et al. (2007) Science 318, 1917-20.

原厂资料：

Specificity / Sensitivity

Nanog (D1G10) Rabbit mAb (Mouse Specific; ChIP Formulated) immunoprecipitates endogenous levels of total mouse nanog protein. This antibody does not cross-react with human nanog protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein.

Background

Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3).

1. Kim, J. et al. (2008) Cell 132, 1049-61.
2. Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
3. Yu, J. et al. (2007) Science 318, 1917-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.