MitoTracker? Deep Red FM

• 产品编号：CST-8778S      品牌：CST       原厂货号：8778S
• 产品分类：生化和细胞分析 > 标签、标记试剂盒 > 荧光配基
• 应用分类：

描述：

每小瓶MitoTracker® Deep Red FM包含50 μg冻干的固体。对于1 mM浓度贮存液，在冻干粉中加入91.98 μl 高品质的DMSO。最佳染色浓度和孵育时间根据应用的不同而有所不同。将贮存液直接用生长培养基稀释到终浓度为500nm，37ºC培养30分钟后，可获得典型的结果。培养过后，将细胞用冰冷的100%甲醇在-20ºC固定20分钟，再用PBS洗3次，每次5分钟。然后开始进行免疫染色。MitoTracker® dyes是一种细胞可透过的探针，包含一个轻度巯基活性的氯甲基部分来进行线粒体的标记。为了标记线粒体，只需将细胞与MitoTracker® probes一起培养，然后探针就会被动扩散，穿过质膜，在有活性的线粒体中积聚。

原厂资料：

Directions for Use

Each vial of MitoTracker® Deep Red FM contains 50 μg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 91.98 μl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results can be obtained by diluting the stock solution directly in growth media to a final concentration of 500 nM and incubating for 30 min at 37ºC. After incubation, fix the cells in ice-cold, 100% methanol for 15 min at -20ºC and rinse 3 times with PBS for 5 min. Proceed with immunostaining.

Background

MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.

注意事项：

Storage: Store lyophilized solid at -20ºC dessicated and
protected from light. In lyophilized form, this reagent is stable
for 6 months. Once reconstituted in DMSO, the solution can be
stored at -20ºC, protected from light and must be used within
2 weeks. Avoid freeze-thaw cycles.