PTBP1 Antibody

• 产品编号：CST-8776S      品牌：CST       原厂货号：8776S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PTBP1 Antibody能够检测内源性的 PTBP1总蛋白水平。该多克隆抗体是经由合成的对应于人PTBP10蛋白 Val338 位点周围序列的肽段免疫动物而生产的。经过Protein A和亲和层析纯化。PTBP1/hnRNP I属于heterogeneous nuclear ribonucleoproteins (hnRNPs)家族成员，它们可在RNA代谢的许多方面发挥多种功能（1）。PTBP1对于调控可变剪接、IRES介导的翻译起始以及提高多种转录本的稳定性都是至关重要的（2-4）。最近研究发现，PTBP1可与来自20S蛋白酶体的PSMA3产生相互作用，并且参与蛋白降解过程（5,6）。PTBP1广泛表达，并能在核和胞质见穿梭。PTBP1的细胞定位和它与多种因子的相互作用决定了PTBP1功能的多样性（4）。

1. Chen, M. and Manley, J.L. (2009) Nat Rev Mol Cell Biol 10, 741-54.
2. Amir-Ahmady, B. et al. (2005) RNA 11, 699-716.
3. Gama-Carvalho, M. et al. (2006) Genome Biol 7, R113.
4. Sawicka, K. et al. (2008) Biochem Soc Trans 36, 641-7.
5. Lee, K.A. et al. (2011) J Biol Chem 286, 41530-8.
6. Fedorova, O.A. et al. (2011) Biochem Biophys Res Commun 416, 258-65.

原厂资料：

Specificity / Sensitivity

PTBP1 Antibody recognizes endogenous levels of total PTBP1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val338 of human PTBP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

PTBP1/hnRNP I belongs to the family of heterogeneous nuclear ribonucleoproteins (hnRNPs) that exert diverse functions in multiple aspects of RNA metabolism (1). PTBP1 is critical for regulating alternative splicing, IRES-mediated translation initiation, and increased stability of a variety of transcripts (2-4). Recently, PTBP1 was found to interact with PSMA3 from the 20S proteasome and is therefore implicated in the process of protein degradation (5,6). PTBP1 is ubiquitously expressed and shuttles between the nucleus and cytoplasm. Cellular localization of PTBP1 and its interactions with various factors determines the diversity of PTBP1 function (4).

1. Chen, M. and Manley, J.L. (2009) Nat Rev Mol Cell Biol 10, 741-54.
2. Amir-Ahmady, B. et al. (2005) RNA 11, 699-716.
3. Gama-Carvalho, M. et al. (2006) Genome Biol 7, R113.
4. Sawicka, K. et al. (2008) Biochem Soc Trans 36, 641-7.
5. Lee, K.A. et al. (2011) J Biol Chem 286, 41530-8.
6. Fedorova, O.A. et al. (2011) Biochem Biophys Res Commun 416, 258-65.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.