DYRK1A (D30C10) Rabbit mAb

• 产品编号：CST-8765S      品牌：CST       原厂货号：8765S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DYRK1A (D30C10) Rabbit mAb 兔单抗能够检测内源性的DYRK1A总蛋白。在一些细胞系中该抗体可能会检测到~65 kDa或蛋白~28 kDa的条带。该单克隆抗体是由合成的人源DYRK1A蛋白天冬酰胺(523位)肽段免疫动物而制备的。DYRK家族包括双重特异性酪氨酸磷酸化及可调节的激酶，可以磷酸化酪氨酸和丝氨酸/苏氨酸残基（1）。DYRK家族通过酵母Yak1（2）和果蝇minibrain（MNB）激酶（3）确定其同源性。哺乳动物中已经发现七个亚型，包括DYRK1A，DYRK1B，DYRK1C，DYRK2，DYRK3，DYRK4，DYRK4B。DYRK家族具有底物特异性、表达、亚细胞定位等差异（4,5）。所有DYRK蛋白质在催化区域的激活环都有一个Tyr-X-Tyr基序，第二个酪氨酸是磷酸化的（如DYRK1A Tyr312），这是激酶活性所必需的。DYRKs通常能够自身磷酸化其激活环上的酪氨酸残基，但却磷酸化底物上的丝氨酸和苏氨酸残基（1,6）。DYRK1A磷酸化RPX(S/T)P保守序列中的丝氨酸和苏氨酸残基。其底物包括FoxO1和cAMP反应元件结合蛋白（如NFAT）等转录因子（7,8）。DYRK1A在胎儿和成人组织中广泛表达。多拷贝DYRK1A转基因小鼠表现出学习和运动缺陷，这表明它是一个剂量敏感的基因（9）。DYRK1A基因定位于染色体21q22.2，该区域与唐氏综合症有关，可能有助于观察21号染色体三体综合征的病理特征（10）。

1. Becker, W. and Joost, H.G. (1999) Prog. Nucleic Acid Res. Mol. Biol. 62, 1-17.
2. Garrett, S. and Broach, J. (1989) Genes Dev. 3, 1336-1348.
3. Tejedor, F. et al. (1995) Neuron 14, 287-301.
4. Kentrup, H. et al. (1996) J. Biol. Chem. 271, 3488-3495.
5. Becker, W. et al. (1998) J. Biol. Chem. 273, 25893-25902.
6. Lochhead, P.A. et al. (2005) Cell 121, 925-936.
7. von Groote-Bidlingmaier, F. et al. (2003) Biochem Biophys Res Commun 300, 764-9.
8. Gwack, Y. et al. (2006) Nature 441, 646-50.
9. Altafaj, X. et al. (2001) Hum Mol Genet 10, 1915-23.
10. Guimera, J. et al. (1999) Genomics 57, 407-18.

原厂资料：

Specificity / Sensitivity

DYRK1A (D30C10) Rabbit mAb recognizes endogenous levels of total DYRK1A protein. This antibody may also recognize a background band at ~65 kDa or ~28 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn523 of human DYRK1A protein.

Background

The DYRK family includes several dual-specificity tyrosine-phosphorylated and regulated kinases capable of phosphorylating proteins at both Tyr and Ser/Thr residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the Drosophila minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop; phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) is necessary for kinase activity. DYRKs typically autophosphorylate the Tyr residue within their activation loop, but phosphorylate substrates at Ser and Thr residues (1,6). DYRK1A phosphorylates serine and threonine residues within an RPX(S/T)P consensus sequence. Substrates include transcription factors, such as FoxO1 and cAMP response element-binding proteins, such as NFAT (7,8). DYRK1A is ubiquitously expressed in fetal and adult tissues. Transgenic mice with multiple copies of DYRK1A exhibit learning and motor defects, suggesting that it is a dosage-sensitive gene (9). The DYRK1A gene localizes to chromosome 21q22.2, a region implicated by research studies in Down syndrome, and may contribute to pathological traits observed in chromosome 21 trisomy (10).

1. Becker, W. and Joost, H.G. (1999) Prog. Nucleic Acid Res. Mol. Biol. 62, 1-17.
2. Garrett, S. and Broach, J. (1989) Genes Dev. 3, 1336-1348.
3. Tejedor, F. et al. (1995) Neuron 14, 287-301.
4. Kentrup, H. et al. (1996) J. Biol. Chem. 271, 3488-3495.
5. Becker, W. et al. (1998) J. Biol. Chem. 273, 25893-25902.
6. Lochhead, P.A. et al. (2005) Cell 121, 925-936.
7. von Groote-Bidlingmaier, F. et al. (2003) Biochem Biophys Res Commun 300, 764-9.
8. Gwack, Y. et al. (2006) Nature 441, 646-50.
9. Altafaj, X. et al. (2001) Hum Mol Genet 10, 1915-23.
10. Guimera, J. et al. (1999) Genomics 57, 407-18.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.