A2B5 Mouse mAb

• 产品编号：CST-8763S      品牌：CST       原厂货号：8763S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

A2B5 Mouse mAb鼠单抗识别内源性的A2B5抗原。A2B5 Mouse mAb鼠单抗已经通过验证，用于推荐的染色流程。染色前固定细胞将导致伪影。基于已发表文献，预测该抗体会与鸡、大鼠、牛和人源样品发生交叉反应。该单克隆抗体是由鸡胚视网膜细胞免疫动物而制备的。A2B5 Mouse mAb鼠单抗识别的细胞表面神经节苷脂抗原表位，已被用作标记物来鉴定各种细胞类型。A2B5 Mouse mAb鼠单抗已被用于标记特定的细胞群，如神经内分泌细胞、胸腺上皮细胞（1）和产生于II型星形胶质细胞和少突胶质细胞的神经胶质前体（2-4）。

1. Botham, C.A. et al. (2001) Cell Tissue Res 303, 381-9.
2. Eisenbarth, G.S. et al. (1979) Proc Natl Acad Sci U S A 76, 4913-7.
3. Sontheimer, H. et al. (1990) Proc Natl Acad Sci U S A 87, 9833-7.
4. Bottenstein, J.E. et al. (1988) J Neurosci Res 20, 291-303.

原厂资料：

Specificity / Sensitivity

A2B5 Mouse mAb recognizes endogenous levels of A2B5 antigen. A2B5 Mouse mAb has been validated for use with the recommended staining protocol. Fixation of cells prior to staining will lead to artifacts. Based on literature publications, this antibody is predicted to cross-react with chicken, rat, bovine, and human samples.

Source / Purification

Monoclonal antibody is produced by immunizing animals with embryonic chicken retinal cells.

Background

A2B5 Mouse mAb recognizes a cell surface ganglioside epitope that has been utilized as a marker for identification of various cell types. A2B5 Mouse mAb has been used to mark specific cell populations such as neuroendocrine cells, thymic epithelial cells (1), and glial precursors that give rise to type II astrocytes and oligodendrocytes (2-4).

标记特定的细胞群，如神经内分泌细胞、胸腺上皮细胞（1）和产生于II型星形胶质细胞和少突胶质细胞的神经胶质前体（2-4）。

1. Botham, C.A. et al. (2001) Cell Tissue Res 303, 381-9.
2. Eisenbarth, G.S. et al. (1979) Proc Natl Acad Sci U S A 76, 4913-7.
3. Sontheimer, H. et al. (1990) Proc Natl Acad Sci U S A 87, 9833-7.
4. Bottenstein, J.E. et al. (1988) J Neurosci Res 20, 291-303.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.