SMARCB1/BAF47 (D9C2) Rabbit mAb

• 产品编号：CST-8745S      品牌：CST       原厂货号：8745S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SNF5 (D9C2) Rabbit mAb兔单抗能够检测内源性SNF5总蛋白水平。该单克隆抗体是采用合成的与人源SNF5蛋白Gln244残基周围序列相对应的肽段免疫动物而生产的。

ATP依赖的染色质修饰复合物在调节细胞核进程中起着重要的作用，例如转录、DNA复制和修复(1,2)。SWI/SNF染色质修饰复合物是有超过10个亚单位组成，并且包含BRM或BRG1的一个单独分子，这两个蛋白作为ATPase催化亚单位。ATPase亚单位的激活干扰组蛋白-DNA相互作用，并且改变了染色质重要调节元件的接近能力。另外中心的和附加的亚单位起到支撑作用去维持稳定性，并且提供了界面为不同转录因子和染色质相互作用(2-5)。SWI/SNF亚单位和转录因子例如细胞核受体、p53、Rb、BRCA1、MyoD之间的相互作用有助于复合物招募到靶基因去调节基因激活、细胞生长、细胞周期和分化进程(1,6-9)。

SNF5是SWI/SNF复合物中心亚单位之一，该蛋白在体外对于由BRG1引起高效核小体重组是必须的(10)。SNF5是esBAF(mouse embryonic stem cell specific SWI/SNF complex)的一个重要部分，并且对于早期胚胎发育和肝细胞分化是必须的(11,12)。因此，SNF5被认为是一个肿瘤抑制蛋白；非活性的突变已经被确定在大量的恶性杆状的肿瘤中(13,14)。

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
11. Klochendler-Yeivin, A. et al. (2000) EMBO Rep 1, 500-6.
12. Gresh, L. et al. (2005) EMBO J 24, 3313-24.
13. Versteege, I. et al. (1998) Nature 394, 203-6.
14. Biegel, J.A. et al. (1999) Cancer Res 59, 74-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Chicken, Xenopus, Bovine

Specificity / Sensitivity

SNF5 (D9C2) Rabbit mAb recognizes endogenous levels of total SNF5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln244 of human SNF5 protein.

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors such as nuclear receptors, p53, Rb, BRCA1, and MyoD facilitate recruitment of the complex to target genes to regulate gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).

SNF5, one of the core subunits of the SWI/SNF complex, is necessary for efficient nucleosome remodeling by BRG1 in vitro (10). SNF5 is an essential part of the esBAF (mouse embryonic stem cell specific SWI/SNF complex) and is necessary for early embryogenesis and hepatocyte differentiation (11,12). In addition, SNF5 is considered to be a tumor suppressor protein; inactivating mutations have been indentified in a large number of malignant rhabdoid tumors (13,14).

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Phelan, M.L. et al. (1999) Mol Cell 3, 247-53.
11. Klochendler-Yeivin, A. et al. (2000) EMBO Rep 1, 500-6.
12. Gresh, L. et al. (2005) EMBO J 24, 3313-24.
13. Versteege, I. et al. (1998) Nature 394, 203-6.
14. Biegel, J.A. et al. (1999) Cancer Res 59, 74-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.