蛋白G磁珠是一种亲和基体，能小规模地分离免疫沉淀（IP试验）中的免疫复合物。蛋白G能共价结合无孔顺磁性粒子。蛋白G对来自许多物种（包括人、兔、小鼠、大鼠和绵羊）的IgG子类都有较高的亲和力，并且能与这些抗体一起进行免疫沉淀试验。使用我们的6-Tube Magnetic Separation Rack #7017能将小珠从溶液中分离出来，把小珠集中到管子侧壁而不是底部。这样就减去了离心步骤，减少了样品损失，提高了洗涤效率，并节省了时间。每200 μL裂解物中加入40 μL珠浆。将含有磁性小珠的管子放到磁分离架上，等待10-15秒使小珠在磁体附近集中，并等待溶液变清。轻轻地涡旋以重悬溶液。请参考我们的免疫沉淀方案利用磁分离，以获得完整的细节。
原厂资料：
Description
Protein G Magnetic Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein G is covalently coupled to a nonporous paramagnetic particle. Protein G exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies. Beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017, which concentrates the beads to the side of the tube instead of the bottom. This eliminates centrifugation steps, minimizes sample loss, increases washing efficiency, and saves time.
Directions for Use
Use 40 μL of bead slurry per 200 μL lysate. Place tubes containing the magnetic beads in a magnetic separation rack and wait 10-15 seconds for the beads to concentrate near the magnet and for the solution to clear. To resuspend, vortex gently. Please refer to our Immunoprecipitation Protocol Utlilizing Magnetic Separation, for complete protocol details.
注意事项：
Storage: Supplied in a 1% Benzyl alcohol solution.
Store at 4ºC.
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