CISH (D4D9) Rabbit mAb

• 产品编号：CST-8731S      品牌：CST       原厂货号：8731S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CISH (D4D9) Rabbit mAb能够检测内源CISH蛋白总体水平。此单克隆抗体是通过合成人源对应的CISH Pro176位点周围的肽段来免疫动物而获得。SOCS 家族中至少由8个成员组成，包括最初鉴定的CIS1蛋白 (细胞因子诱导的含有SH2域的蛋白)和从SOCS1到SOCS7 七个蛋白。每个SOCS 家族成员包含一个SH2中心域和C-末端域命名为SOCS盒子。这些蛋白是细胞因子信号的重要调控者包括细胞增殖、分化和免疫反应。CISH/CIS1, 是SOCS家族中最初发现的成员, 它能够被包括IL-2, IL-3, GM-CSF 和 EPO在内的一系列细胞因子激活 (4)。CISH蛋白呈现出双分子量，32 和 37 kDa, 这个原因还不清楚(4)。CISH 结合到磷酸化细胞因子受体并抑制Stat5活性(4-6)。CISH的表达由Stat5调控, 属于反馈调控模式(5)。转基因小鼠过表达CISH的表型与Stat5敲除的小鼠表型相似, 包括乳腺发育缺陷和T细胞，NK细胞调控的缺陷(6)。研究表明CISH的多态性与感染疾病的易感性有关(7)。

1. Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
2. Chen, X.P. et al. (2000) Immunity 13, 287-90.
3. Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
4. Yoshimura, A. et al. (1995) EMBO J 14, 2816-26.
5. Matsumoto, A. et al. (1997) Blood 89, 3148-54.
6. Matsumoto, A. et al. (1999) Mol Cell Biol 19, 6396-407.
7. Khor, C.C. et al. (2010) N Engl J Med 362, 2092-101.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

CISH (D4D9) Rabbit mAb recognizes endogenous levels of total CISH protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro176 of human CISH protein.

Background

The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway (1-3). The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses.CISH/CIS1, the first described member of the SOCS family, is induced by a number of cytokines including IL-2, IL-3, GM-CSF, and EPO (4). The CISH protein appears as a doublet around 32 and 37 kDa, the nature of which is unknown (4). CISH binds to phosphorylated cytokine receptors and can inhibit Stat5 activity (4-6). Expression of CISH is regulated by Stat5, thereby providing feedback modulation (5). Transgenic mice overexpressing CISH display phenotypes similar to Stat5 knockouts, including defects in mammary gland development and in T and NK cell regulation (6). Research studies have shown that polymorphisms within the CISH gene are associated with susceptibility to infectious diseases (7).

1. Alexander, W.S. et al. (1999) J Leukoc Biol 66, 588-92.
2. Chen, X.P. et al. (2000) Immunity 13, 287-90.
3. Hilton, D.J. et al. (1998) Proc Natl Acad Sci USA 95, 114-9.
4. Yoshimura, A. et al. (1995) EMBO J 14, 2816-26.
5. Matsumoto, A. et al. (1997) Blood 89, 3148-54.
6. Matsumoto, A. et al. (1999) Mol Cell Biol 19, 6396-407.
7. Khor, C.C. et al. (2010) N Engl J Med 362, 2092-101.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.