SUV39H1 (D11B6) Rabbit mAb

• 产品编号：CST-8729S      品牌：CST       原厂货号：8729S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SUV39H1 (D11B6) Rabbit mAb兔单抗能够检测内源性SUV39H1总蛋白水平。该抗体不与SUV39H2蛋白发生交叉反应。通过合成的与人源SUV39H1蛋白Asp380位点相应的多肽片段去免疫动物从而制备出此单克隆抗体。人源组蛋白甲基转移酶SUV39H1是果蝇显性PEV modifier Su(var)3-9的同系物，其是由412氨基酸残基组成(1)。它能结合从进化程度上染色质调节因子两个保守的区域：chromo和SET区域(2,3)。60氨基酸chromo区域表现出一个古老的组蛋白样折叠，该折叠指导异源染色质的定位。研究证明130氨基酸SET区域包含甲基转移酶催化结构域，该结构协调邻近半胱氨酸富含区去增强组蛋白甲基转移酶活性(1)。该酶选择性使histone H3蛋白Lys9位点甲基化，这可产生一个HP1蛋白结合位点，而HP1是一个异源染色质受体分子家族，其涉及基因沉默和核小体染色质结构(4,5)。SUV39H1组蛋白甲基转移酶在组蛋白氨基端的修饰和基因表达的调节中起着重要作用。

1. Rea, S. et al. (2000) Nature 406, 593-599.
2. Koonin, E. V. et al. (1995) Nucleic Acids Res. 23, 4229-4232.
3. Jenuwein, T. et al. (1998) Cell. Mol. Life Sci. 54, 80-93.
4. Lachner, M. et al. (2001) Nature 410, 116-120.
5. Bannister, A. J. et al. (2001) Nature 410, 120-123.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

SUV39H1 (D11B6) Rabbit mAb recognizes endogenous levels of total SUV39H1 protein. This antibody does not cross-react with the SUV39H2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp380 of human SUV39H1 protein.

Background

Human histone methyltransferase SUV39H1 is the homolog of the dominant Drosophila PEV modifier Su(var)3-9 and is composed of 412 amino acid residues (1). It combines two of the most evolutionarily conserved domains of the "chromatin regulators": the chromo and SET domains (2,3). The 60 amino acid chromo domain represents an ancient histone-like fold that directs heterochromatic localizations. It has been demonstrated that the 130 amino acid SET domain contains the methyltransferase catalytic motif, which cooperates with the adjacent cysteine-rich regions to confer histone methyltransferase activity (1). This enzyme selectively methylates histone H3 on Lys9, which generates a binding site for HP1 proteins, a family of heterochromatic adaptor molecules involved in both gene silencing and supra-nucleosomal chromatin structure (4,5). SUV39H1 histone methyltransferase plays an important role in modification of histone amino termini and regulation of gene expression.

1. Rea, S. et al. (2000) Nature 406, 593-599.
2. Koonin, E. V. et al. (1995) Nucleic Acids Res. 23, 4229-4232.
3. Jenuwein, T. et al. (1998) Cell. Mol. Life Sci. 54, 80-93.
4. Lachner, M. et al. (2001) Nature 410, 116-120.
5. Bannister, A. J. et al. (2001) Nature 410, 120-123.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.