MAP2 (D5G1) XP? Rabbit mAb

• 产品编号：CST-8707P      品牌：Cell Signaling Technology       原厂货号：8707P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MAP2 (D5G1) XP® Rabbit mAb兔单抗能够检测内源性的MAP2总蛋白。该单克隆抗体是由合成的人源MAP2蛋白C末端肽段免疫动物而制备的。微管相关蛋白2（MAP2）是一种神经元磷酸化蛋白，调节轴突和树突中微管的结构和稳定性，神经元的形态，细胞骨架动力学和细胞器的运输（1）。在神经元中MAP2表达多种亚型，其中包括超高分子量的MAP2A和MAP2B（280和270 kDa）以及低分子量的MAP2C和MAP2D（70和75 kDa）。MAP2的磷酸化调节其与细胞骨架的结合，并受到发育的调控。GSK3和p44/42 MAP激酶磷酸化MAP2的丝氨酸136，苏氨酸1620和苏氨酸1623（2,3）。GSK3对苏氨酸1620/1623的磷酸化抑制MAP2与微管蛋白的结合，减小微管稳定性（3）。

1. Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
2. Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
3. Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

MAP2 (D5G1) XP® Rabbit mAb recognizes endogenous levels of total MAP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAP2 protein.

Background

Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3).

1. Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
2. Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
3. Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.