LIN28A (D9F5) Rabbit mAb

• 产品编号：CST-8706S      品牌：CST       原厂货号：8706S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LIN28A (D9F5)兔源单克隆抗体识别内源性的总LIN28A蛋白水平。此单克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与包围于人源LIN28A蛋白邻近羧基末端的氨基酸残基序列一致。LIN28A 和LIN28B是一类在发育过程中保守的调控RNA结合蛋白，它抑制let-7家族的miRNAs的处理加工和成熟过程[1,2]。let-7 miRNAs已经显示抑制ras、myc和Hmga2等癌基因[3]。近来研究显示LIN28A 和LIN28B在人类主要肿瘤和肿瘤细胞系中上调表达。它们的过表达直接与下调的let-7 miRNAs正相关[4]。LIN28基因被报道与原生殖细胞发育和精原细胞恶性肿瘤相关[5]。LIN28B的等位基因变异与人类青春期周期调控有关[6]。过表达的LIN28A联合Oct-4, Sox2, 和Nanog可重调人成纤维细胞重编程成多潜能ES样细胞[7]。

1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
3. Cho, W.C. (2007) Mol Cancer 6, 60.
4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
5. West, J.A. et al. (2009) Nature 460, 909-13.
6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
7. Yu, J. et al. (2007) Science 318, 1917-20.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey

Specificity / Sensitivity

LIN28A (D9F5) Rabbit mAb recognizes endogenous levels of total LIN28A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human LIN28A protein.

Background

LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).

1. Balzer, E. and Moss, E.G. (2007) RNA Biol 4, 16-25.
2. Piskounova, E. et al. (2008) J Biol Chem 283, 21310-4.
3. Cho, W.C. (2007) Mol Cancer 6, 60.
4. Viswanathan, S.R. et al. (2009) Nat Genet 41, 843-8.
5. West, J.A. et al. (2009) Nature 460, 909-13.
6. Ong, K.K. et al. (2009) Nat Genet 41, 729-33.
7. Yu, J. et al. (2007) Science 318, 1917-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.