SignalSilence? FoxO3a siRNA II (Mouse Specific)

• 产品编号：CST-8702S      品牌：CST       原厂货号：8702S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

FoxO3a siRNA II (Mouse Specific）抑制小鼠和大鼠 FoxO3a的表达。cst公司SignalSilence ® FoxO3a siRNA II (小鼠特异性)使得研究者可以通过使用ＲＮＡ干扰来特异性抑制tFoxO3的表达。RNA干扰是一种通过向细胞中输入双链RNA分子来选择性的抑制基因的表达的方法。CST公司的所有SignalSilence ® siRNA 产品已经过严格的内部测试，western分析显示其可以降低靶蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST建议用100 nM SignalSilence ® FoxO3a siRNA II (Mouse Specific)转染48-72 小时后裂解细胞。对于转染步骤，参见转染试剂生产商的说明书。如果有问题请随时与ＣＳＴ联系。Forkhead box（Fox）蛋白是一类进化保守的转录因子家族，因其存在翼状螺旋的DNA结合结构域而得名（1）。人类中存在超过40种已知的Fox蛋白家族成员，分为19个亚家族，已演化成能在动物发育过程中广泛且高度特异地调节基因的转录（2）。干扰Fox家族成员表达的突变最终被发现与一系列人类疾病包括免疫功能异常、不育、演讲/语言障碍和肿瘤有密切联系（3,4）。

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
4. Benayoun, B.A. et al. (2011) Trends Genet 10, 224-32.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

FoxO3a siRNA II (Mouse Specific) inhibits mouse and rat FoxO3a expression.

Description

SignalSilence® FoxO3a siRNA II (Mouse Specific) from Cell Signaling Technology (CST) allows the researcher to specifically inhibit FoxO3a expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® FoxO3a siRNA II (Mouse Specific) 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout animal development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4).

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
4. Benayoun, B.A. et al. (2011) Trends Genet 10, 224-32.

注意事项：

Storage: FoxO3a siRNA II (Mouse Specific) is supplied in
RNAse-free water. Aliquot and store at -20ºC