Cleaved Caspase Substrate Motif [DE(T/S/A)D] MultiMab? Rabbit mAb mix

• 产品编号：CST-8698S      品牌：CST       原厂货号：8698S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cleaved Caspase Substrate Motif [DE(T/S/A)D] Rabbit mAb兔单抗能够检测内源性的酶切过的带有羧基端天冬氨酸残基的caspase 蛋白水平，很少情况下，可以检测到带有羧基端谷氨酸残基的caspase蛋白水平。该抗体不会与未酶切过的完整蛋白或者羧基末端带有不同残基的蛋白产生交叉反应。单克隆抗体是由合成的羧基端含有天冬氨酸基序的多肽库免疫动物制备。该抗体由多个单克隆抗体组成，含有四个克隆以产生广泛的反应性。凋亡是一个受到高度调控、细胞程序化死亡的生理过程，是多种细胞组织生长和发育的一种正常组成部分。Caspase 是天冬胺酸介导的半胱氨酸蛋白酶，是凋亡机制的中枢（1）。这个内在的通路能够从来源于细胞内的信号如DNA损伤启动凋亡级联反应。而外在的通路能够对细胞外的信号如FasL响应，启动凋亡。在内在和外在通路中，起始因子caspase包括多种效应分子caspase以及细胞死亡的初级执行者 caspase-3会酶切下游底物（2,3）。效应器caspase会放大凋亡级联反应调控许多对正常细胞功能至关重要的靶蛋白。除了在发育生物学中的作用，凋亡调控对于研究癌症、自身免疫疾病以及传染性疾病具有广泛影响（4）。成千上万的已知或推测的caspase酶切位点呈现在人类蛋白质组中，几乎都含有天冬氨酸残基位点的酶切，同样也有在Glu残基位点的酶切，但很少(5)。

原厂资料：

Specificity / Sensitivity

Cleaved Caspase Substrate Motif [DE(T/S/A)D] Rabbit mAb recognizes endogenous levels of caspase-cleaved proteins with a carboxy-terminal aspartic acid residue, and in rare cases a carboxy-terminal glutamic acid residue . This antibody does not cross-react with whole proteins or those ending with a different carboxy-terminal residue.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing a carboxy-terminal Asp motif. The antibody is multiclonal, containing four clones to elicit broad reactivity.

Background

Apoptosis is a physiological process resulting in a highly regulated, programmed form of cell death that is a normal part of growth and development in multicellular organisms. Caspases are aspartic acid-directed cysteine proteases that are central to the apoptotic mechanism (1). The intrinsic pathway initiates an apoptotic cascade from signals originating within the cell, such as DNA damage, while an extrinsic pathway initiates apoptosis in response to extracellular signals, like FasL. In both intrinsic and extrinsic pathways, initiator caspases cleave downstream substrates, including multiple effector caspases and the primary executioner of cell death, caspase-3 (2,3). Effector caspases amplify the apoptotic cascade to target many critical proteins needed for normal cell function. Apart from its role in developmental biology, the regulation of apoptosis has broad implications for the study of cancer, autoimmune disorders and infectious diseases (4). Thousands of known and putative caspase cleavage sites are present within the human proteome; almost all sites involve cleavage at an aspartic acid residue, though cleavage at glutamic acid residues is seen, rarely, as well (5).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.