Angiogenesis Antibody Sampler Kit

• 产品编号：CST-8696S      品牌：Cell Signaling Technology       原厂货号：8696S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

血管生成抗体小包装组合试剂盒中的每一个抗体能够识别其特定靶点的磷酸化形式。磷酸化FAK (Tyr397) (D20B1) 兔单抗可能与过表达的酪氨酸磷酸化蛋白如EGFR，发生交叉反应。磷酸化Src Family (Tyr416) (D49G4)兔单抗可能与其他Src家族成员或过表达的磷酸化RTKs发生交叉反应。磷酸化VEGFR2 (Tyr1175) (19A10) 兔单抗可能与VEGFR1发生交叉反应。多克隆抗体通过用合成磷酸肽免疫动物制备，该合成磷酸肽是人Akt蛋白Ser473、人FAK蛋白Tyr397、人p38 MAPK蛋白Thr180/Tyr182、人p44 MAPK蛋白Thr202/Tyr204、人PLCγ1蛋白Ser1248、人Src蛋白Tyr416、人VEGFR2蛋白Tyr1175附近的残基。Angiogenesis Antibody Sampler Kit提供了一种调查血管通路下游VEGFR2的经济手段。该试剂盒包含足够完成四次western blot实验的一抗。血管内皮细胞生长因子受体2（VEGFR2，KDR，Flk-1）是内皮细胞中VEGF诱导信号的主要受体。 VEGFR2与配体结合后，发生自身磷酸化和激活（1）。VEGFR2信号是体内血管生成所必需的（2-4）。受体的激活导致衔接蛋白的快速招募，包括Shc，GRB2，PI3K激酶，NCK，蛋白酪氨酸磷酸酶SHP-1和SHP-2（5）。VEGFR2 Tyr1212的磷酸化为GRB2在Tyr1175结合和磷酸化PI3K激酶的p85亚基和PLCγ提供了一个停靠位点（1,5,6）。在血管生成中VEGFR2的激活导致信号通过多个下游激酶途径，包括Akt，Src，FAK，p38和Erk1 / 2（2,7）。

原厂资料：

Specificity / Sensitivity

Each antibody in the Angiogenesis Antibody Sampler Kit recognizes the phosphorylated form of its specific target. Phospho-FAK (Tyr397) (D20B1) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR. Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb may cross-react with other Src family members or overexpressed phosphorylated RTKs. Phospho-VEGFR2 (Tyr1175) (19A10) Rabbit mAb may cross-react with VEGFR1.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser473 of human Akt protein, Tyr397 of human FAK protein, Thr180/Tyr182 of human p38 MAPK protein, Thr202/Tyr204 of human p44 MAPK protein, Ser1248 of human PLCγ1 protein, Tyr416 of human Src protein, and Tyr1175 of human VEGFR2 protein.

Description

The Angiogenesis Antibody Sampler Kit provides an economical means to investigate the angiogenic pathway downstream of VEGFR2. The kit contains enough primary antibody to perform four western blots per primary antibody.

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Signaling from VEGFR2 is necessary for angiogenesis in vivo (2-4). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (5). Phosphorylation of VEGFR2 at Tyr1212 provides a docking site for GRB2 binding and phosphorylation at Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ (1,5,6). Activation of VEGFR2 during angiogenesis leads to signaling through multiple downstream kinase pathways including Akt, Src, FAK, p38, and Erk1/2 (2,7).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.