Lysine Acetyltransferase Antibody Sampler Kit

产品编号：CST-8686S 品牌：Cell Signaling Technology 原厂货号：8686S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
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包装：

1Kit

运保温度:

–20°C.

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其它组分：

GCN5L2 (C26A10) Rabbit mAb 【子货号：#3305，包装：40 µl，，运保温度：–20°C.】

PCAF (C14G9) Rabbit mAb 【子货号：#3378，包装：40 µl，，运保温度：–20°C.】

Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody 【子货号：#4771，包装：40 µl，，运保温度：–20°C.】

Anti-rabbit IgG, HRP-linked Antibody 【子货号：#7074，包装：100 µl，，运保温度：–20°C.】

CBP (D6C5) Rabbit mAb 【子货号：#7389，包装：40 µl，，运保温度：–20°C.】

描述：

Lysine Acetyltransferase Antibody Sampler Kit中的每种抗体能够识别各自的内源性的目的蛋白。该抗体不会与其他家族成员产生交叉反应。单克隆抗体经由合成的人CBP蛋白、人GCN5L2蛋白或人PCAF蛋白的氨基端氨基酸多肽免疫动物而生产的。多克隆抗体是经由合成的围绕人CBP蛋白的Lys1535残基的氨基酸多肽免疫动物而生产的。多克隆抗体经由蛋白质A和多肽亲和层析纯化而成。Lysine Acetyltransferase Antibody Sampler Kit可以提供一种快速经济的方法来检测内源性赖氨酸乙酰转移酶的水平，包括Acetyl-CBP, CBP, GCN5L2, 和 PCAF。试剂盒提供的每种一抗都足够进行4次western blot实验。

原厂资料：

Specificity / Sensitivity

Each antibody in the Lysine Acetyltransferase Antibody Sampler Kit recognizes endogenous levels of respective target protein. The antibodies do not cross-react with other family members.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human CBP protein, human GCN5L2 protein, or human PCAF protein. Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Lysine Acetyltransferase Antibody Sampler Kit provides an economical means to examine several lysine acetyltrasferases, including: Acetyl-CBP, CBP, GCN5L2, and PCAF. The kit contains enough primary antibody to perform four western blots per primary.

Background

CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). The role of acetylation of CBP/p300 is of particular interest (2,3). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (4). p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B) (5), and General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) (6) are transcriptional adaptor proteins in addition to HATs. PCAF functions as the catalytic subunit of the PCAF transcriptional co-activator complex (5). GCN5L2 functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (6). PCAF and GCN5L2 acetylate histone H3 at Lys14 and histone H4 at Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (7,8). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD) and general transcription factors (TFIIEβ and TFIIF) (9-12). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (13,14), transcription co-activators (PGC1-α) (15), and nuclear receptors (Steroidogenic Factor 1) (16). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (13-16).

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibody.