Oncogene and Tumor Suppressor Antibody Sampler Kit

• 产品编号：CST-8678S      品牌：Cell Signaling Technology       原厂货号：8678S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Phospho-Akt (Ser473) (D9E) XP® 兔 mAb可以识别473位丝氨酸被磷酸化的内源性Akt。BRCA1抗体可以识别内源性总的BRCA1蛋白。该抗体可以识别BRCA1的核亚型1,2和4，但是不能识别BRAC1细胞质亚型3和5，也不能与BRCA2反应。E-Cadherin (24E10)兔mAb可以识别内源性总的E-Cadherin 蛋白。该抗体与其他的家族成员，例如N-cadherin没有交叉反应。Phospho-Estrogen Receptor α (Ser167) (D1A3)兔mAb可以识别167位丝氨酸被磷酸化的内源性ERα。该抗体有一条月77kDa的非特异性条带。HER2/ErbB2 (D8F12) XP®Rabbit mAb可以识别内源性总的HER2/ErbB2蛋白。p53 (7F5)Rabbit mAb可以识别内源性总的p53蛋白。PTEN (138G6)Rabbit mAb可以识别内源性总的PTEN蛋白。Stathmin可以识别内源性总的stathmin蛋白。该抗体与其他相关蛋白，例如SCG10, SCLIP, and RB3，没有交叉反应。磷酸特异性单抗由合成肽段免疫动物产生，该肽段分别与人Akt蛋白473位丝氨酸附近氨基酸或人雌激素受体α167位丝氨酸附近氨基酸序列一致。单抗由合成肽段免疫动物产生，肽段分别与人E-cadherin蛋白780脯氨酸，人HER2/ErbB2蛋白氨基端，人PTEN蛋白羧基端或人p53蛋白全长一致。多抗也是由合成的肽段免疫动物产生，肽段分别与人BRCA1蛋白按极端或人stathmin蛋白38位丝氨酸邻近氨基酸序列一致。多抗由蛋白A和肽段亲和层析技术纯化得到。癌基因和肿瘤抑制抗体取样试剂盒提供了一种经济的方法去研究癌症发生，肿瘤浸润和癌变病理过程涉及到信号通路及蛋白。试剂盒提供了足以进行4次western blot实验的一抗和二抗。肿瘤发生是涉及到多个步骤，会导致若干原癌基因，抑癌基因和micorRNA基因的突变（1，2）。这些突变通常会破坏调控细胞生长和程序性死亡的一些蛋白的表达，功能，并且/或者活性。已经证实了多个调控肿瘤发生和癌变病理的分子机制及生物通路。在肿瘤发生的过程中，调控凋亡，细胞周期进程，细胞黏附，细胞转移和DNA修复反应的通路都会被破坏。HER2/ErbB2 (3), E-Cadherin (4), p53 (5,6), Stathmin (7), BRCA1 (8,9), Akt (10), PTEN (11), 和 Estrogen Receptor α (12)在这些通路中发挥了重要作用。

原厂资料：

Specificity / Sensitivity

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb recognizes endogenous levels of Akt only when phosphorylated at Ser473. BRCA1 Antibody recognizes endogenous levels of total BRCA1 protein. The antibody detects BRAC1 nuclear isoforms 1, 2, and 4, but not BRAC1 cytoplasmic isoforms 3 and 5, and does not detect BRCA2. E-Cadherin (24E10) Rabbit mAb recognizes endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb recognizes endogeneous levels of ERα only when phosphorylated at Ser167. The antibody cross reacts with a nonspecific band at around 77 kDa. HER2/ErbB2 (D8F12) XP® Rabbit mAb recognizes endogenous levels of total HER2/ErbB2 protein. p53 (7F5) Rabbit mAb detects endogenous levels of total p53 protein. The antibody binding has been mapped to the amino terminus region of human p53 protein. PTEN (138G6) Rabbit mAb recognizes endogenous levels of total PTEN protein. Stathmin Antibody recognizes endogenous levels of total stathmin protein. The antibody does not cross-react with related proteins, such as SCG10, SCLIP, and RB3.

Source / Purification

Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser473 of human Akt protein or Ser167 of human estrogen receptor α protein. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein, residues near the amino terminus of human HER2/ErbB2 protein, the carboxy terminus of human PTEN protein, or with a full-length human p53 fusion protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human BRCA1 protein or residues surrounding Ser38 of human stathmin protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Oncogenes and Tumor Suppressor Antibody Sampler Kit offers an economical means of investigating proteins commonly involved in the biological pathways behind oncogenesis, tumor metastasis, and cancer pathology. The kit contains enough primary and secondary antibody to perform four western blot experiments with each antibody.

Background

Oncogenesis is a multistep process leading to sequential alterations in several oncogenes, tumor-suppressor genes, and microRNA genes (1,2). These alterations often disrupt the expression, function, and/or activity of proteins regulating cell growth and programmed cell death. Many of the molecular mechanisms and biological pathways driving oncogenesis and cancer pathology have been identified. The signal transduction pathways regulating apoptosis, cell-cycle progression, cell adhesion, cell migration, and DNA damage responses are often disrupted. HER2/ErbB2 (3), E-Cadherin (4), p53 (5,6), Stathmin (7), BRCA1 (8,9), Akt (10), PTEN (11), and Estrogen Receptor α (12) function in many of these pathways

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibody