Mitochondrial Marker Antibody Sampler Kit

• 产品编号：CST-8674S      品牌：Cell Signaling Technology       原厂货号：8674S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

每个Mitochondrial Marker Antibody Sampler Kit试剂盒中的抗体均识别其对应的蛋白水平。SOD1 (71G8) Mouse mAb与其它相关蛋白无交叉反应。HSP60 (D307)抗体与其它HSP蛋白没有交叉反应。兔单克隆抗体用与人类COX IV中Lys29位点、人类细胞色素c中67-78氨基酸残基、人丙酮酸脱氢酶序列或人VDAC-1蛋白氨基末端附近的氨基酸序列对应的人工合成肽段免疫动物制成。鼠单克隆抗体用与全长人类SOD1蛋白免疫动物制得。多克隆抗体用与人类HSP60蛋白Asp307、人类PHB1蛋白中Ser252或人SDHA蛋白羧基末端附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化制得。Mitochondrial Marker Antibody Sampler Kit提供了一个经济的手段来研究线粒体相关的蛋白。该试剂盒包含足量的使得每个一抗完成四次Western blots的量。Mitochondrial Marker Antibody Sampler Kit包含多个用来识别已确认的线粒体蛋白的抗体。细胞色素c氧化酶（COX）是一个异寡聚酶，由13个亚基组成，定位于线粒体内膜（1）。细胞色素c是一个非常保守的电子转运蛋白，是定位于线粒体膜间隙中的呼吸链的一部分（2）。HSP60主要被认为是线粒体蛋白，对关键蛋白质转入线粒体后的正确折叠很重要（3）。在线粒体中，prohibitins (PHB1)主要作为膜结合的环复合物存在，以分子伴侣的形式在蛋白质合成和转运的过程中保持线粒体蛋白的稳定性（4）。在哺乳动物细胞中，丙酮酸脱氢酶复合体位于线粒体基质中（5）。琥珀酸脱氢酶（SDH），又称复合物II或琥珀酸醌还原酶，是三羧酸循环和电子传递链中的重要组成部分（6）。SOD1广泛表达，定位于细胞质、细胞核和线粒体膜间隙（7）。电压依赖的阴离子通道（VDAC），广泛表达，定位于线粒体外膜，被认为是代谢产物在线粒体内外弥散的主要途径（8）。

原厂资料：

Specificity / Sensitivity

Each antibody in the Mitochondrial Marker Antibody Sampler Kit recognizes endogenous levels of total respective protein. SOD1 (71G8) Mouse mAb does not cross-react with other related proteins. HSP60 (D307) Antibody does not cross-react with other HSPs.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein, residues 67-78 of human cytochrome c protein, the sequence of human pyruvate dehydrogenase protein, or residues near the amino terminus of human VDAC-1 protein. Mouse monoclonal antibody is produced by immunizing animals with full-length recombinant human SOD1 protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp307 of human HSP60 protein, residues surrounding Ser252 of human PHB1 protein, or residues near the carboxy terminus of human SDHA protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Mitochondrial Marker Antibody Sampler Kit provides an economical means to evaluate relevant mitochondial proteins. This kit contains enough primary antibody to perform four western blots per primary.

Background

The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1). Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (4). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (5). Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (6). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space (7). Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (8).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.