Phospho-SGTA (Ser305) (D23E10) Rabbit mAb

• 产品编号：CST-8664S      品牌：CST       原厂货号：8664S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb兔单抗可识别内源性的Ser305磷酸化的SGTA水平。该单克隆抗体用与人类SGTA蛋白中Ser305位点附近的氨基酸序列对应的人工合成磷酸化肽段免疫动物而制成。SGTA（小的富含谷氨酸tetratricopeptide重复序列的蛋白A)，是广泛表达的分子伴侣，可以直接结合HSC70和HSP70，调节它们的ATP酶活性（1,2）。SGTA是一个34kDa的蛋白质，在羧基末端富含谷氨酸残基，包含三个串联重复TPR基序（3）。SGTA的TPR区域显示出与Hop、CHIP和TOM70中TPR区域的序列相似性（4）。SGTA的TPR区域也能和HSP90相互作用，近来也被发现是一种促凋亡因子（5,6）。SGTA的Ser305磷酸化位点由CST公司使用PhosphoScan®技术鉴定的，这个技术是CST的基于LC-MS/MS的磷酸化发现平台（7）。位点特异性的突变分析表明Ser305的磷酸化对于PDGFRα的稳定和PDGFRα依赖的癌细胞生存至关重要（7）。

1. Liu, F.H. et al. (1999) J Biol Chem 274, 34425-32.
2. Tobaben, S. et al. (2003) J Biol Chem 278, 38376-83.
3. Cziepluch, C. et al. (1998) J Virol 72, 4149-56.
4. Scheufler, C. et al. (2000) Cell 101, 199-210.
5. Liou, S.T. and Wang, C. (2005) Arch Biochem Biophys 435, 253-63.
6. Yin, H. et al. (2006) Biochem Biophys Res Commun 343, 1153-8.
7. Moritz, A. et al. (2010) Sci Signal 3, ra64.

原厂资料：

Specificity / Sensitivity

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb recognizes endogenous levels of SGTA protein only when phosphorylated at Ser305.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser305 of human SGTA protein.

Background

SGTA, small glutamine-rich tetratricopeptide repeat-containing protein A, is an ubiquitously expressed co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity (1,2). SGTA is a 34 kDa protein that is rich in glutamine residues at its C terminus and contains three tandemly repeated TPR motifs (3). The TPR domain of SGTA shows sequence similarity to the TPR domains of Hop, CHIP, and TOM70 (4). The TPR domain of SGTA also interacts with HSP90 and was recently found to be a pro-apoptotic factor (5,6).Phosphorylation of SGTA at Ser305 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (7). Site-specific mutation analysis indicated that phosphorylation at Ser305 is essential for PDGFR α stabilization and PDGFR α-dependent cancer cell survival (7).

1. Liu, F.H. et al. (1999) J Biol Chem 274, 34425-32.
2. Tobaben, S. et al. (2003) J Biol Chem 278, 38376-83.
3. Cziepluch, C. et al. (1998) J Virol 72, 4149-56.
4. Scheufler, C. et al. (2000) Cell 101, 199-210.
5. Liou, S.T. and Wang, C. (2005) Arch Biochem Biophys 435, 253-63.
6. Yin, H. et al. (2006) Biochem Biophys Res Commun 343, 1153-8.
7. Moritz, A. et al. (2010) Sci Signal 3, ra64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.