SignalSilence? hnRNP LL siRNA II

• 产品编号：CST-8624S      品牌：CST       原厂货号：8624S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® hnRNP LL siRNA II 能够抑制人、小鼠以及猴子中hnRNP LL 的表达。Cell Signaling Technology （CST）的SignalSilence^® hnRNP LL siRNA II 可以通过RNA干扰特异性抑制 hnRNP LL的表达。RNA干扰是一种通过传递双链RNA分子到细胞中从而选择性沉默目的基因表达的方法。所有SignalSilence^® siRNA 产品均经过严格的内部测试并通过Western验证可有效降低目的蛋白的表达。通过三苯基分析对寡核苷酸合成进行逐个碱基的监测以保证适当的耦合效率，随后通过亲和固相提取来纯化寡核苷酸。退火的双链RNA需要通过质谱进行进一步的分析从而确定提取物的组成。为了保证批次间一致性的最大化，每一批次产品都要通过质谱与前一批次的产品进行比较。CST建议使用 100 nM SignalSilence^® hnRNP LL siRNA II 进行转染，并在48-72小时后裂解细胞。具体的转染操作步骤请根据转染试剂生产商提供的方法进行。如您在使用过程中有任何问题请随时联系我们。每瓶中含有100次转染所需用量。每次转染siRNA的终浓度为100 nM，以上用量为24孔板中转染，每孔总体积为300 μl。Heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL)是一个细胞核内RNA结合蛋白，它与hnRNP L蛋白共有69% 氨基酸同源序列，hnRNP L是hnRNP复合物的组分，能够调控mRNA生成，包装以及加工(1)。 hnRNP LL蛋白在活化的T细胞中被诱导，并且可作为对T细胞发育和激活至关重要的酪氨酸磷酸酶CD45选择性剪接的关键调控因子(2)。hnRNP LL 还能够调控CD44 和Stat5a的选择性剪接(2)。hnRNP LL有四个异构体，都是通过选择性剪接产生的，并且在人类组织中广泛表达(3)。

1. Pinol-Roma, S. et al. (1989) J Cell Biol 109, 2575-87.
2. Oberdoerffer, S. et al. (2008) Science 321, 686-91.
3. Shur, I. et al. (2004) Gene 334, 113-21.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Monkey

Specificity / Sensitivity

SignalSilence^® hnRNP LL siRNA II inhibits human, mouse, and monkey hnRNP LL expression.

Description

SignalSilence^® hnRNP LL siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit hnRNP LL expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence^® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence^® hnRNP LL siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Heterogeneous nuclear ribonucleoprotein L-like (hnRNP LL) is a nuclear RNA-binding protein that shares 69% amino acid homology with hnRNP L, a component of the hnRNP complex that regulates mRNA formation, packaging, and processing (1). hnRNP LL is induced in activated T cells and functions as a critical regulator of alternative splicing of CD45, a tyrosine phosphatase crucial for T cell development and activation (2). hnRNP LL also regulates the splicing of CD44 and Stat5a (2). The four isoforms of hnRNP LL are generated by alternative splicing and are widely expressed in human tissues (3).

1. Pinol-Roma, S. et al. (1989) J Cell Biol 109, 2575-87.
2. Oberdoerffer, S. et al. (2008) Science 321, 686-91.
3. Shur, I. et al. (2004) Gene 334, 113-21.

注意事项：

Storage: hnRNP LL siRNA II is supplied in RNAse-free water.
Aliquot and store at -20ºC