Transketolase Antibody

• 产品编号：CST-8616S      品牌：CST       原厂货号：8616S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Transketolase（转酮酶）抗体识别内源性的转酮酶总蛋白水平。该多克隆抗体用与人类转酮酶中Val296位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化制得。转酮酶（TKT），是磷酸戊糖途径（PPP）中的一个二聚体，催化酮糖和醛糖的转化（1,2）。此酶与转醛酶一起，与PPP糖酵解的非氧化分支联系（1-3）。TKT的一些区域在进化上与革兰阴性的细菌和哺乳动物相保守（3）。有证据表明缺氧（4）和非缺氧诱导的HIF1-α（5）增加TKT的表达。因为癌细胞依靠TKT进行核酸的补救合成，因此已有研究开始将TKT抑制剂作为一种新型抗癌治疗手段（5-8）。

1. Mitschke, L. et al. (2010) J Biol Chem 285, 31559-70.
2. Esakova, O.A. et al. (2005) Life Sci 78, 8-13.
3. Schenk, G. et al. (1997) J Mol Evol 44, 552-72.
4. Haseloff, R.F. et al. (2006) Proteomics 6, 1803-9.
5. Zhao, F. et al. (2010) Oncogene 29, 2962-72.
6. Thomas, A.A. et al. (2008) Bioorg Med Chem Lett 18, 2206-10.
7. Thomas, A.A. et al. (2008) Bioorg Med Chem Lett 18, 509-12.
8. Raïs, B. et al. (1999) FEBS Lett 456, 113-8.

原厂资料：

Specificity / Sensitivity

Transketolase Antibody recognizes endogenous levels of total transketolase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val296 of human transketolase protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Transketolase (TKT) is a homodimer of the pentose phosphate pathway (PPP) that catalyzes the interketol transfer between ketoses and aldoses (1,2). This enzyme, along with transaldolase, connects the nonoxidative branch of the PPP with glycolysis (1-3). Several regions of TKT are evolutionarily conserved from gram-negative bacteria to mammals (3). There is evidence that hypoxic (4) and non-hypoxic induction of HIF1-α (5) increases the expression of TKT. Because cancer cells rely on TKT in altered cell metabolism for nucleic acid synthesis, work has been done to develop inhibitors of TKT as novel cancer treatments (5-8).

1. Mitschke, L. et al. (2010) J Biol Chem 285, 31559-70.
2. Esakova, O.A. et al. (2005) Life Sci 78, 8-13.
3. Schenk, G. et al. (1997) J Mol Evol 44, 552-72.
4. Haseloff, R.F. et al. (2006) Proteomics 6, 1803-9.
5. Zhao, F. et al. (2010) Oncogene 29, 2962-72.
6. Thomas, A.A. et al. (2008) Bioorg Med Chem Lett 18, 2206-10.
7. Thomas, A.A. et al. (2008) Bioorg Med Chem Lett 18, 509-12.
8. Raïs, B. et al. (1999) FEBS Lett 456, 113-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.