MDH2 Antibody

• 产品编号：CST-8610S      品牌：CST       原厂货号：8610S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MDH2 Antibody 用于检测内源性苹果酸脱氢酶2(MDH2)总蛋白水平。该多抗通过用与人MDH2蛋白C端序列相同的合成肽免疫动物制备而来。抗体的纯化采用蛋白A与肽段亲和层析相结合的方法。苹果酸脱氢酶是三羧酸循环和苹果酸/天冬氨酸穿梭过程中的关键酶(1,2)。在大多数细菌和几乎所有的真核生物中，MDH都有表达(2)。胞质的MDH同工酶(cMDH或者MDH1)主要是在苹果酸/天冬氨酸穿梭过程中，将草酰乙酸转化成苹果酸(1,2,3)。而线粒体中MDH的同工酶(mMDH或MDH2)的作用主要是在三羧酸循环中将苹果酸转化成草酰乙酸(1,2)。

1. Matsuda, T. et al. (2010) J Pharmacol Sci 112, 320-6.
2. Minárik, P. et al. (2002) Gen Physiol Biophys 21, 257-65.
3. Joh, T. et al. (1987) J Biol Chem 262, 15127-31.

原厂资料：

Specificity / Sensitivity

MDH2 Antibody recognizes endogenous levels of total MDH2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MDH2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid cycle and malate/aspartate shuttle (1,2). MDH is widely expressed in organisms from most bacteria to all eukaryotes (2). The cytoplasmic MDH isoenzyme (cMDH or MDH1) primarily reduces oxaloacetate to malate in the malate/aspartate shuttle (1,2,3). The major function of mitochondrial MDH isoenzyme (mMDH or MDH2) is to oxidize malate to oxaloacetate in the tricarboxylic acid cycle (1,2).

1. Matsuda, T. et al. (2010) J Pharmacol Sci 112, 320-6.
2. Minárik, P. et al. (2002) Gen Physiol Biophys 21, 257-65.
3. Joh, T. et al. (1987) J Biol Chem 262, 15127-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.