SINTBAD (D1A5) Rabbit mAb

• 产品编号：CST-8605S      品牌：CST       原厂货号：8605S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SINTBAD (D1D5) Rabbit mAb兔单抗能够检测内源SINTBAD蛋白总体水平。此单克隆抗体是通过合成人源对应的SINTBAD Leu55位点周围的肽段来免疫动物而获得。SINTBAD, 在与其同源的 NAP1蛋白基础上得到鉴定, 它是两个在天然免疫响应中发挥重要作用的两个激酶—— TBK1 和 IKKε的接头蛋白(1)。这些激酶能够引起磷酸化并接下来激活转录因子IRF-3, 这是免疫响应基因调控所需要的(2)。SINTBAD 激活TBK1 和 IKKε的精确机制尚不清楚。表达SINTBAD蛋白的中间TBD (TBK 结合区域)区域能发挥一个区域性失活蛋白的作用，并能干扰IRF-3的激活。SINTBAD 和 NAP1 也能与NDP52蛋白结合, 并和一些多泛素化蛋白结合，比如在细菌表面产生的蛋白，并能激活细胞的自噬作用(3)。

1. Ryzhakov, G. and Randow, F. (2007) EMBO J 26, 3180-90.
2. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
3. Thurston, T.L. et al. (2009) Nat Immunol 10, 1215-21.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine

Specificity / Sensitivity

SINTBAD (D1D5) Rabbit mAb recognizes endogenous levels of total SINTBAD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu55 of human SINTBAD protein.

Background

SINTBAD, identified based on homology to NAP1, is an adaptor protein for TBK1 and IKKε, two related kinases that play a pivotal role in innate immune response (1). These kinases trigger the phosphorylation and subsequent activation of the transcription factor IRF-3, resulting in gene regulation required for immune responses (2). The precise mechanism by which SINTBAD activates TBK1 and IKKε is still unclear. Expression of a central region of SINTBAD termed a TBD (TBK binding domain) can function as a dominant negative protein and interfere with IRF-3 activation. SINTBAD and NAP1 can also bind to NDP52, a protein that associates with poly-ubiquitinated proteins, such as those produced on the surfaces of bacteria, and can trigger autophagy (3).

1. Ryzhakov, G. and Randow, F. (2007) EMBO J 26, 3180-90.
2. Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
3. Thurston, T.L. et al. (2009) Nat Immunol 10, 1215-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.