Dopamine β-Hydroxylase (DBH) Antibody

• 产品编号：CST-8586S      品牌：CST       原厂货号：8586S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Dopamine β-Hydroxylase (DBH) Antibody 兔多抗识别内源性的总DBH蛋白。该多克隆抗体由合成的人类 dopamine β-hydroxylase中Ala211位点附近肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。多巴胺β-羟化酶（DBH）是铜II型抗坏血酸依赖的单加氧酶家族中的一种酶。这种酶由两个非共价二硫键将两个二聚体相连，有膜结合和可溶性形式（1-3）。可溶性形式在管腔内的分泌颗粒中出现（4），并以细胞胞吐释放（5）。DBH将多巴胺转换为去甲肾上腺素（6）这种酶的缺乏会导致一种罕见的疾病，特点是血浆中完全没有去甲肾上腺素和肾上腺素，伴有多巴胺的水平增加（7）。体位性低血压是DBH的主要症状，可通过注射人工合成的去甲肾上腺素前体二羟基苯丝氨酸来治疗（8）。

1. Smith, W.J. and Kirshner, N. (1967) Mol Pharmacol 3, 52-62.
2. Lagercrantz, H. (1976) Neuroscience 1, 81-92.
3. Winkler, H. (1976) Neuroscience 1, 65-80.
4. Laduron, P.M. (1975) FEBS Lett 52, 132-4.
5. Weinshilboum, R.M. et al. (1971) Science 174, 1349-51.
6. Kaufman, S. and Friedman, S. (1965) Pharmacol Rev 17, 71-100.
7. Robertson, D. et al. (1986) N Engl J Med 314, 1494-7.
8. Biaggioni, I. and Robertson, D. (1987) Lancet 2, 1170-2.

原厂资料：

Specificity / Sensitivity

Dopamine β-Hydroxylase (DBH) Antibody recognizes endogenous levels of total DBH protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala211 of human dopamine β-hydroxylase protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Dopamine β-Hydroxylase (DBH) is an enzyme of the copper type II ascorbate-dependent mono-oxygenase family. This enzyme forms homotetramers composed of two noncovalently bound disulfide-linked dimers and is found as both membrane-associated and soluble forms (1-3). The soluble form is present in the lumen of secretory granules (4) and is released from cells by exocytosis (5). DBH converts dopamine to noradrenaline (6). Deficiency in this enzyme causes a rare disease characterized by a complete absence of noradrenaline and adrenaline in plasma together with increased plasma dopamine levels (7). Orthostatic hypotension, the main symptom of DBH deficiency, can be alleviated by administration of dihydroxyphenylserine, a synthetic precursor of noradrenaline (8).

1. Smith, W.J. and Kirshner, N. (1967) Mol Pharmacol 3, 52-62.
2. Lagercrantz, H. (1976) Neuroscience 1, 81-92.
3. Winkler, H. (1976) Neuroscience 1, 65-80.
4. Laduron, P.M. (1975) FEBS Lett 52, 132-4.
5. Weinshilboum, R.M. et al. (1971) Science 174, 1349-51.
6. Kaufman, S. and Friedman, S. (1965) Pharmacol Rev 17, 71-100.
7. Robertson, D. et al. (1986) N Engl J Med 314, 1494-7.
8. Biaggioni, I. and Robertson, D. (1987) Lancet 2, 1170-2.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.