APR3 Antibody

• 产品编号：CST-8581S      品牌：CST       原厂货号：8581S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

APR3 Antibody 能够检测内源 APR3 总蛋白。该多克隆抗体是采用合成的人源APR3(a 形式)蛋白 Pro29 位点周围残基相对应的肽段来免疫动物而获得。抗体是通过protein A 和多肽亲和层析法纯化。凋亡相关蛋白3 (APR3)，也被称做 C2orf28，最初是在经过反式视黄酸处理HL-60细胞中得到鉴定的膜蛋白，随后发现在其它的敏感细胞系中APR3能够被ATRA诱导 (1,2)。APR3 也能够被NFAT和NF-κB活性所上调表达(3)。被ATRA所调控的现象表明，APR3 在细胞分化的过程中发挥作用, 但是其中的作用机理尚不清楚。APR3的过表达能够通过诱导细胞周期停在G1/S 期，并下调 cyclin D1的表达，从而抑制细胞的增值 (2)。APR3 也能够与NELL-1 作用而调控造骨细胞的分化(4)。

1. Zhu, F. et al. (2000) Biotechniques 29, 310-3.
2. Yu, F. et al. (2007) Biochem Biophys Res Commun 358, 1041-6.
3. Yang, G. et al. (2007) Mol Cell Biochem 302, 187-94.
4. Zou, X. et al. (2011) FEBS Lett 585, 2410-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

APR3 Antibody recognizes endogenous levels of total APR3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro29 of human APR3 protein (isoform a). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Apoptosis related protein 3 (APR3), also known as C2orf28, is a membrane protein identified in HL-60 cells treated with all-trans retinoic acid (ATRA) and was later found to be induced by ATRA in other sensitive cell lines (1,2). APR3 is also up-regulated by NFAT and NF-κB activities (3). Regulation of APR3 by ATRA suggests a role in cell differentiation, but the mechanism of action is still unclear. Overexpression of APR3 can inhibit proliferation by inducing G1/S cell cycle arrest and decreasing expression of cyclin D1 (2). APR3 has also been shown to interact with NELL-1 to regulate osteoblast differentiation (4).

1. Zhu, F. et al. (2000) Biotechniques 29, 310-3.
2. Yu, F. et al. (2007) Biochem Biophys Res Commun 358, 1041-6.
3. Yang, G. et al. (2007) Mol Cell Biochem 302, 187-94.
4. Zou, X. et al. (2011) FEBS Lett 585, 2410-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody