Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb

• 产品编号：CST-8578S      品牌：CST       原厂货号：8578S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Acetyl-CoA Carboxylase 2 (D5B9)兔单克隆抗体识别内源性的乙酰辅酶A羧化酶2总蛋白水平。该单克隆抗体用与人类乙酰辅酶A羧化酶2蛋白中Val1416位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。乙酰辅酶A羧化酶（ACC）催化丙乙酰辅酶A羧化为丙二酰辅酶A（1）。这是脂肪酸生物合成和氧化的关键酶（1）。在啮齿类动物中，265 kDa ACC1 (ACCα)主要表达在生脂组织中，280 kDa ACC2 (ACCβ)主要表达在氧化组织中（1,2）。但是，人类中ACC2在生脂和氧化组织中都是主要的（1,2）。AMPK可以磷酸化其Ser79，PKA可以磷酸化其Ser1200，均可抑制ACC的酶活性（3）。ACC是一个抗肥胖药物的潜在靶点（4,5）。

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

原厂资料：

Specificity / Sensitivity

Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb recognizes endogenous levels of total acetyl-CoA carboxylase 2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1416 of human acetyl-CoA carboxylase 2 protein.

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while the 280 kDa ACC2 (ACCβ) form is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody