GOPC (D10A12) Rabbit mAb

• 产品编号：CST-8576S      品牌：CST       原厂货号：8576S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GOPC (D10A12) Rabbit mAb兔单抗识别内源性的GOPC总蛋白。该单克隆抗体用与人类GOPC蛋白中Phe31位点附近的氨基酸序列对应的人工合成肽段免疫动物而制成。GOPC（FIG）首先被识别为与高尔基体结合的包含PDZ结构域的蛋白。它两个卷曲螺旋结构域（CC1和CC2）定位于氨基末端区域，一个PDZ结构域定位于羧基末端区域（1）。CC2结构域和其邻近的连接区介导GOPC与高尔基蛋白golgin-160和Q-SNARE蛋白连接子6的结合（1,2）。GOPC的PDZ结构域与目标蛋白的羧基末端相互作用，介导目标蛋白囊泡转运和表面表达（3-6）。已经在一些胶质瘤中检测到对应的GOPC和ROS酪氨酸激酶癌融合基因。产生的GOPC-ROS融合蛋白被转运至高尔基体，在那里组成性激活的ROS酪氨酸激酶可以介导肿瘤生成（7,8）。

1. Charest, A. et al. (2001) J Biol Chem 276, 29456-65.
2. Hicks, S.W. and Machamer, C.E. (2005) J Biol Chem 280, 28944-51.
3. Cheng, J. et al. (2002) J Biol Chem 277, 3520-9.
4. He, J. et al. (2004) J Biol Chem 279, 50190-6.
5. Wente, W. et al. (2005) J Biol Chem 280, 32419-25.
6. Ito, H. et al. (2006) Biochem J 397, 389-98.
7. Charest, A. et al. (2003) Proc Natl Acad Sci USA 100, 916-21.
8. Charest, A. et al. (2006) Cancer Res 66, 7473-81.

原厂资料：

Specificity / Sensitivity

GOPC (D10A12) Rabbit mAb detects endogeneous levels of total GOPC protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe31 of human GOPC protein.

Background

GOPC (FIG) was originally identified as a Golgi-associated, PDZ domain containing protein. It has two coiled-coil domains (CC1 and CC2) located in the amino-terminal region and a PDZ domain in the carboxy-terminal region (1). The CC2 domain and its adjacent linker region mediate the association of GOPC with the golgi protein golgin-160 and the Q-SNARE protein syntaxin 6 (1,2). The PDZ domain of GOPC interacts with the carboxy terminus of target proteins to mediate target protein vesicular trafficking and surface expression (3-6). Fusion of the corresponding GOPC gene with the ROS tyrosine kinase oncogene has been detected in some glioblastomas. The resulting GOPC-ROS fusion protein is targeted to the golgi apparatus where a constitutively activate ROS tyrosine kinase can mediate tumor formation (7,8).

1. Charest, A. et al. (2001) J Biol Chem 276, 29456-65.
2. Hicks, S.W. and Machamer, C.E. (2005) J Biol Chem 280, 28944-51.
3. Cheng, J. et al. (2002) J Biol Chem 277, 3520-9.
4. He, J. et al. (2004) J Biol Chem 279, 50190-6.
5. Wente, W. et al. (2005) J Biol Chem 280, 32419-25.
6. Ito, H. et al. (2006) Biochem J 397, 389-98.
7. Charest, A. et al. (2003) Proc Natl Acad Sci USA 100, 916-21.
8. Charest, A. et al. (2006) Cancer Res 66, 7473-81.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody