MYPT1 (D6C1) Rabbit mAb

• 产品编号：CST-8574S      品牌：CST       原厂货号：8574S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MYPT1 (D6C1)Rabbit mAb可以识别内源性总的MYPT1 蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人MYPT1蛋白羧基端附近氨基酸一致。蛋白质磷酸水解酶1（PP1）是一种广泛表达的真核蛋白丝氨酸/苏氨酸磷酸酶，涉及到多种细胞功能的调控。底物特异性由PP1催化亚基（PP1c）的结合决定。已经证明存在超过50种不同的调控亚基（1）。肌凝蛋白磷酸酶全酶由3个亚基组成：PP1c，一个靶点/调控亚基（肌凝蛋白磷酸酶的MYPT/myosin结合亚基），和一个未知功能的20kDa亚基（M20）。MYPT与PP1cδ结合会改变催化结构区的构象增加酶活和特异性（2）。两个MYPT亚型有61%的相似性。MYPT表达广泛，但是MYPT2似乎特异性地不在心脏和大脑中表达（3）。相关的家族成员包括MBS85, MYPT3, 和 TIMAP (4). 
肌球蛋白磷酸酶在GTPase Rho的刺激下调控肌动蛋白丝和肌球蛋白的结合。Rho通过Rho相关激酶（ROCK）抑制肌球蛋白磷酸酶。MYPT1的696位苏氨酸和853位苏氨酸磷酸化会导致磷酸酶功能抑制和细胞骨架的重构（5,6）。

1. Cohen, P.T. (2002) J Cell Sci 115, 241-56.
2. Terrak, M. et al. (2004) Nature 429, 780-4.
3. Fujioka, M. et al. (1998) Genomics 49, 59-68.
4. Ito, M. et al. (2004) Mol Cell Biochem 259, 197-209.
5. Birukova, A.A. et al. (2004) Microvasc Res 67, 64-77.
6. Birukova, A.A. et al. (2004) J Cell Physiol 201, 55-70.

原厂资料：

Specificity / Sensitivity

MYPT1 (D6C1) Rabbit mAb recognizes endogenous levels of total MYPT1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MYPT1 protein.

Background

Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1). 
 The myosin phosphatase holoenzyme is composed of three subunits: PP1c, a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase), and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed, while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members include MBS85, MYPT3, and TIMAP (4). 
 Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6).

1. Cohen, P.T. (2002) J Cell Sci 115, 241-56.
2. Terrak, M. et al. (2004) Nature 429, 780-4.
3. Fujioka, M. et al. (1998) Genomics 49, 59-68.
4. Ito, M. et al. (2004) Mol Cell Biochem 259, 197-209.
5. Birukova, A.A. et al. (2004) Microvasc Res 67, 64-77.
6. Birukova, A.A. et al. (2004) J Cell Physiol 201, 55-70.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.