Rad17 (D3G6) Rabbit mAb

• 产品编号：CST-8561S      品牌：CST       原厂货号：8561S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rad17 (D3G6) Rabbit mAb能够检测内源性Rad17总蛋白。该单克隆抗体是由合成的人源的针对Rad17蛋白的羧基末端肽段免疫动物生产的。人的检验点蛋白Rad17和其裂变和芽殖酵母的同源基因(裂殖酵母和酿酒酵母中分别为Rad17和Rad24)参与响应DNA损伤或DNA合成破坏的检验点信号激活（1-4）。基因毒性药物处理人体细胞能够诱导ATM/ATR依赖的RAD17的Ser635和Ser645磷酸化。Rad17磷酸化在DNA损伤细胞的检验点信号中是一个重要的早期事件（5-7）。

1. Griffiths, D.J. et al. (1995) EMBO J 14, 5812-23.
2. Li, L. et al. (1999) Oncogene 18, 1689-99.
3. Bao, S. et al. (1998) Cell Growth Differ 9, 961-7.
4. von Deimling, F. et al. (1999) Hum Genet 105, 17-27.
5. Bao, S. et al. (2001) Nature 411, 969-74.
6. Post, S. et al. (2001) Proc Natl Acad Sci U S A 98, 13102-7.
7. Wang, X. et al. (2001) Cancer Res 61, 7417-21.

原厂资料：

Specificity / Sensitivity

Rad17 (D3G6) Rabbit mAb recognizes endogenous levels of total Rad17 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rad17 protein.

Background

The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombe Rad17 and Saccharomyces cerevisiae Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7)

1. Griffiths, D.J. et al. (1995) EMBO J 14, 5812-23.
2. Li, L. et al. (1999) Oncogene 18, 1689-99.
3. Bao, S. et al. (1998) Cell Growth Differ 9, 961-7.
4. von Deimling, F. et al. (1999) Hum Genet 105, 17-27.
5. Bao, S. et al. (2001) Nature 411, 969-74.
6. Post, S. et al. (2001) Proc Natl Acad Sci U S A 98, 13102-7.
7. Wang, X. et al. (2001) Cancer Res 61, 7417-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody