TPX2 Antibody

• 产品编号：CST-8559S      品牌：CST       原厂货号：8559S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TPX2 Antibody能够检测内源性TPX2总蛋白。多克隆抗体是由合成的人源的针对TPX2蛋白赖氨酸（420位）的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。Ras家族小GTP酶Ran参与核被膜形成、有丝分裂纺锤体组装和核运输(1,2)。TPX2，活化Ran(RanGTP)的一个靶标，是一种微管核化蛋白。当结合到核输入因子α时TPX2是失活的。RanGTP的活性破坏了这种相互作用，解除了对TPX2的抑制(3)。TPX2结合激活Aurora A激酶并促进其向有丝分裂纺锤体定位(4,5)。有丝分裂过程中的DNA 损伤导致了Aurora A和TPX2相互作用的缺失以及Aurora A激酶的失活(6)。TPX2在胰腺癌细胞中高表达，敲除TPX2后这些细胞对紫杉醇的敏感性增加(7)。

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
3. Gruss, O.J. and Vernos, I. (2004) J Cell Biol 166, 949-55.
4. Kufer, T.A. et al. (2002) J Cell Biol 158, 617-23.
5. Bayliss, R. et al. (2004) Cell Cycle 3, 404-7.
6. Bhatia, P. et al. (2010) Cell Cycle 9, 4592-9.
7. Warner, S.L. et al. (2009) Clin Cancer Res 15, 6519-28.

原厂资料：

Specificity / Sensitivity

TPX2 Antibody recognizes endogenous levels of total TPX2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys420 of human TPX2 protein. Antibodies are purified by protein A and peptide affinity chromatography

Background

The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle, and nuclear transport (1,2). TPX2, a target of active Ran (RanGTP), is a microtubule nucleating protein. TPX2 is inactive when bound to nuclear importin-alpha. RanGTP activity disrupts this interaction, relieving inhibition of TPX2 (3). TPX2 binding activates Aurora A kinase and promotes its localization to the mitotic spindle (4,5). DNA damage in mitosis leads to loss of interaction between Aurora A and TPX2 and inactivation of Aurora A kinase (6). TPX2 is highly expressed in pancreatic cancer cells, and knockdown of TPX2 expression in these cells is associated with increased sensitivity to paclitaxel (7). 

1. Quimby, B.B. and Dasso, M. (2003) Curr Opin Cell Biol 15, 338-44.
2. Hetzer, M. et al. (2002) Nat Cell Biol 4, E177-84.
3. Gruss, O.J. and Vernos, I. (2004) J Cell Biol 166, 949-55.
4. Kufer, T.A. et al. (2002) J Cell Biol 158, 617-23.
5. Bayliss, R. et al. (2004) Cell Cycle 3, 404-7.
6. Bhatia, P. et al. (2010) Cell Cycle 9, 4592-9.
7. Warner, S.L. et al. (2009) Clin Cancer Res 15, 6519-28.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.