Annexin V Antibody

• 产品编号：CST-8555S      品牌：CST       原厂货号：8555S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Annexin V抗体可以识别内源性总的Annexin V蛋白。该孔与其他Annexin V家族成员没有交叉反应。多抗由合成肽段免疫动物产生，该肽段与人annexin V蛋白氨基端附近肽段序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Annexin V，也被称为PAP-1或Lipocortin V，是一个~30kDa蛋白与磷脂质以钙离子-依赖的形式结合（1）。所有的annexins都有一个潜在的PKC结合位点，但是只有annexin V被认为是该通路的抑制子（2）。也可能意味着，直接与VEGFR2受体结合，调控血管内皮细胞的增殖（3）。Annexin V与凝血酶原竞争，优先地与磷脂酰丝氨酸结合，从而导致在损伤部位脂质成分之间的接触和凝血因子启动血栓前抑制血液凝固（4-6）。Annexin V与磷脂酰丝氨酸之间特异的牢固的结合使它成为了一个检测凋亡细胞的重要因素（7）。Annexin V可由糖皮质激素诱导，能够被酪氨酸和丝氨酸/苏氨酸激酶磷酸化（8）。据信，中止生成炎症介导因子，例如前列腺素和白三烯，通过抑制花生四烯酸从膜磷脂酶A2释放（8）。

1. Huber, R. et al. (1990) EMBO J 9, 3867-74.
2. Cardó-Vila, M. et al. (2003) Mol Cell 11, 1151-62.
3. Wen, Y. et al. (1999) Biochem Biophys Res Commun 258, 713-21.
4. Koopman, G. et al. (1994) Blood 84, 1415-20.
5. London, F. et al. (1996) Biochemistry 35, 16886-97.
6. Thiagarajan, P. and Benedict, C.R. (1997) Circulation 96, 2339-47.
7. Vermes, I. et al. (1995) J Immunol Methods 184, 39-51.
8. Grundmann, U. et al. (1988) Proc Natl Acad Sci USA 85, 3708-12.

原厂资料：

Specificity / Sensitivity

Annexin V Antibody recognizes endogenous levels of total annexin V protein. This antibody is not predicted to cross-react with other annexin family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human annexin V protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Annexin V, also known as PAP-1 or Lipocortin V, is a ~30 kDa protein that binds to phospholipids in a calcium-dependent manner (1). All annexins contain a putative PKC binding site, but only annexin V has been identified as an inhibitor of this pathway (2). It may also signal, by direct interaction with VEGFR2 receptor, in the regulation of vascular endothelial cell proliferation (3). Annexin V preferentially binds phosphatidylserine, in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis (4-6). The ability of Annexin V to bind specifically and robustly to phosphatidylserine makes it an attractive reagent in detecting apoptotic cells (7). Annexin V is inducible by glucocorticoids and can be phosphorylated by tyrosine and serine/threonine kinases (8). It is thought to block production of mediators of inflammation, such as prostaglandins and leukotrienes by inhibiting the release of arachidonic acid from membranes by phospholipase A2 (8).

1. Huber, R. et al. (1990) EMBO J 9, 3867-74.
2. Cardó-Vila, M. et al. (2003) Mol Cell 11, 1151-62.
3. Wen, Y. et al. (1999) Biochem Biophys Res Commun 258, 713-21.
4. Koopman, G. et al. (1994) Blood 84, 1415-20.
5. London, F. et al. (1996) Biochemistry 35, 16886-97.
6. Thiagarajan, P. and Benedict, C.R. (1997) Circulation 96, 2339-47.
7. Vermes, I. et al. (1995) J Immunol Methods 184, 39-51.
8. Grundmann, U. et al. (1988) Proc Natl Acad Sci USA 85, 3708-12.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.