PMSF

• 产品编号：CST-8553S      品牌：CST       原厂货号：8553S
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 蛋白抑制剂、酶抑制剂 > 蛋白酶抑制剂
• 应用分类：

描述：

苯甲基磺酰氟（PMSF）是丝氨酸蛋白酶类（如胰蛋白酶、糜蛋白酶、凝血酶和木瓜蛋白酶）的抑制剂。它常作为添加物在裂解前加入裂解缓冲液中，来防止蛋白酶的降解。CST公司建议在Cell Lysis Buffer (#9803) 和RIPA Buffer (#9806)中加入1mM的PMSF。分子式：C7H7FO2S，分子量：174.19，CAS: 329-98-6。对于200mM(200X)浓度贮存液，在34.84 mg PMSF冻干粉中加入1ml异丙醇。PMSF在水中不稳定，应该在使用前加入裂解缓冲液或其他水溶液中。通常来讲，终浓度为1mM的PMSF就能够对蛋白酶提供足够的保护了。冻干粉能在常温下避光保存12个月。一旦溶解，PMSF溶液能在-20ºC避光保存3个月。

原厂资料：

Description

Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation. Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer (#9803) and RIPA Buffer (#9806). Molecular Formula: C7H7FO2S Molecular Weight: 174.19 CAS: 329-98-6

Directions for Use

For a 200 mM (200X) stock solution, reconstitute the 34.84 mg of lyophilized PMSF supplied in the product tube in 1 mL isopropyl alcohol. PMSF is unstable in water and should be added to lysis buffers or other aqueous solutions just prior to use. Typically, a final concentration of 1 mM provides sufficient protease protection. Store lyophilized at RT for 12 months protected from direct light. Once in solution, PMSF can be stored at -20ºC for up to 3 months, protected from light.

注意事项：

Directions for Use: For a 200 mM (200X) stock solution,
reconstitute the 34.84 mg of lyophilized PMSF supplied
in the product tube in 1 mL isopropyl alcohol. PMSF is
unstable in water and should be added to lysis buffers or
other aqueous solutions just prior to use. Typically, a final
concentration of 1 mM provides sufficient protease protec-
tion. Store lyophilized at RT for 12 months protected from
direct light. Once in solution, PMSF can be stored at -20ºC
for up to 3 months, protected from light.