Phospho-Histone H3 (Ser10) (D2C8) XP? Rabbit mAb (Pacific Blue? Conjugate)

• 产品编号：CST-8552S      品牌：CST       原厂货号：8552S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb 兔单抗(Pacific Blue™ Conjugate)能够检测内源性Ser10位点磷酸化的组蛋白H3水平。该抗体与其他磷酸化组蛋白或乙酰化组蛋白没有交叉反应。该单克隆抗体采用合成的与人组蛋白H3蛋白Ser10位点的氨基酸磷酸化肽段免疫动物而生产的。该CST抗体偶联了Pacific Blue™荧光染料。并通过直标法流式细胞术在人细胞内进行内部检测。它与unconjugated Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377兔单抗具有相同的种属交叉反应性。染色质结构调整在真核生物的转录调控中扮演着一个重要角色。核小体是染色质的基本结构单位，由DNA缠绕八个核心组蛋白 (H2A、H2B、H3、H4各2个分子)组成(1)。核心组蛋白的氨基端尾部常发生多种翻译后修饰，包括乙酰化、磷酸化、甲基化和泛素化 (2-5)。这些修饰都是针对不同刺激而产生的反应，且会直接影响染色质对转录因子的可接近性，进而影响基因表达（6）。在多数物种中，组蛋白H2B 乙酰化主要发生在Lys5， 12， 15和20位点上 (4,7)。而组蛋白H3的乙酰化则主要发生在Lys9， 14， 18， 23， 27和56位点上。在一些生物体内，H3 Lys9位点的乙酰化修饰看似对组蛋白沉积和染色质组装有决定性的作用 (2,3)。组蛋白H3 Ser10、Ser28和Thr11位点上的磷酸化修饰与有丝分裂和减数分裂期间的染色体浓缩紧密相关(8-10)。组蛋白H3 Thr3位点上的磷酸化在许多物种中是高度保守的，由激酶haspin催化完成。在哺乳动物细胞内用磷酸化特异性抗体进行免疫染色，发现有丝分裂中H3 Thr3 位点的磷酸化发生在前期，而去磷酸化则发生在后期（11）。

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

原厂资料：

Specificity / Sensitivity

Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of histone H3 only when phosphorylated at Ser10. This antibody does not cross-react with other phosphorylated histones or with acetylated histones.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3 protein.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377.

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
4. Cheung, P. et al. (2000) Cell 103, 263-71.
5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze